DNA Windmill Probe for Multiplexed mRNA Detection and Cell Type Discrimination
DNA Windmill Probe for Multiplexed mRNA Detection and Cell Type Discrimination
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DOI：
10.1002/chem.202301300
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发表时间：
2023-07-28
影响因子：
4.3
通讯作者：
Wang，Chunyan
中科院分区：
文献类型：
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作者：
Zhao，Yu;Li，Zhihao;Wang，Chunyan
准确的肿瘤诊断尤其是早期诊断对及时治疗和提高生存率具有重要意义。mRNA被广泛用作癌症鉴定和治疗的生物标志物。mRNA表达水平与癌症分期和恶性进展高度相关。然而，单一类型的mRNA检测是不够的和不可靠的。在此，我们开发了一种DNA纳米风车探针，用于原位多重mRNA检测和成像。 该探针被设计为通过风叶片同时靶向四种类型的mRNA。重要的是，靶标的识别是彼此独立的，这进一步促进了细胞类型的区分。该探针可以特异性地区分癌细胞系和正常细胞。此外，它还可以识别活细胞中mRNA表达水平的变化。目前的策略丰富了提高癌症诊断和治疗解决方案准确性的工具箱。
Accurate cancer diagnosis especially early diagnosis is of great importance for prompt therapy and elevated survival rate. mRNAs are widely used as biomarkers for cancer identification and treatment. mRNA expression levels are highly associated with cancer stage and malignant progression. Nevertheless, single type mRNA detection is insufficient and unreliable. Herein, we developed a DNA nano‐windmill probe for in situ multiplexed mRNAs detection and imaging in this paper. The probe is designed to simultaneously target four types of mRNA through wind blades. Importantly, recognition of targets is independent from each other, which further facilitate cell type discrimination. The probe can specifically distinguish cancer cell lines from normal cells. In addition, it can identify changes in mRNA expression levels in living cells. The current strategy enriches the toolbox for improving the accuracy of cancer diagnosis and therapeutic solutions.