Effects of oxygen generating scaffolds on cell survival and functional recovery following acute spinal cord injury in rats
Effects of oxygen generating scaffolds on cell survival and functional recovery following acute spinal cord injury in rats
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DOI：
10.1007/s10856-020-06453-y
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发表时间：
2020-11
期刊：
影响因子：
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通讯作者：
Liangle Liu;Junming Wan;M. Dai;Xiuzhi Ye;Chun Liu;Chengxuan Tang;Lixin Zhu
中科院分区：
文献类型：
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作者：
Liangle Liu;Junming Wan;M. Dai;Xiuzhi Ye;Chun Liu;Chengxuan Tang;Lixin Zhu
持续的局部供氧对于创造急性脊髓损伤（SCI）中细胞存活和神经再生的微环境至关重要。本研究的目的是将过氧化钙微球与三维支架结合，作为一种新型的SCI释氧治疗方法。当在缺氧条件下孵育时，支架能够在21天的过程中产生氧气。在体外，将GFP标记的骨髓间充质干细胞（MSCs）植入支架中。我们观察到，支架可以提高MSC在缺氧条件下存活超过21天。将产氧支架植入大鼠脊髓损伤部位。移植后12周，由于组织再生，损伤/移植部位的空腔面积显著减少。此外，通过vWF免疫染色观察到，产氧支架改善了血管再生。一个显著的特征是在损伤部位发生神经纤维再生，这最终导致显著的运动恢复。本研究结果表明，产氧支架具有持续局部释氧的特性，从而促进损伤脊髓的再生。
Persistent local oxygen delivery is crucial to create a microenvironment for cell survival and nerve regeneration in acute spinal cord injury (SCI). This study aimed to fabricate calcium peroxide-based microspheres incorporated into a 3-D construct scaffold as a novel oxygen release therapy for SCI. The scaffolds were able to generate oxygen over the course of 21 days when incubated under hypoxic conditions. In vitro, GFP-labeled bone marrow-derived mesenchymal stem cells (MSCs) were planted into the scaffolds. We observed that scaffolds could enhance MSC survival under hypoxic conditions for more than 21 days. Oxygen generating scaffolds were transplanted into spinal cord injury sites of rats in vivo. Twelve weeks following transplantation, cavity areas in the injury/graft site were significantly reduced due to tissue regeneration. Additionally, the oxygen generating scaffolds improved revascularization as observed through vWF immunostaining. A striking feature was the occurrence of nerve fiber regeneration in the lesion sites, which eventually led to significant locomotion recovery. The present results indicate that the oxygen generating scaffolds have the property of sustained local oxygen release, thus facilitating regeneration in injured spinal cords.