Effects of Electromagnetic Pulse Irradiation on the Mouse Blood-testicle Barrier
Effects of Electromagnetic Pulse Irradiation on the Mouse Blood-testicle Barrier
复制标题
电磁脉冲照射对小鼠血睾屏障的影响
DOI：
10.1016/j.urology.2012.04.015
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发表时间：
2012-07-01
期刊：
影响因子：
2.1
通讯作者：
Zhang, Yuan-Qiang
中科院分区：
文献类型：
--
作者：
Hou, Wu-Gang;Zhao, Jie;Zhang, Yuan-Qiang
目的探讨电磁脉冲辐射对小鼠血-睾丸屏障（blood-testicle barrier，BTB）及精子发生的影响。方法采用400 kV/m电磁脉冲全身照射小鼠，以苏木精-伊红、伊文思蓝、硝酸镧为示踪剂，观察小鼠睾丸及BTB通透性的变化。结果照射后1、7、14 d，BTB结构受损，通透性明显增加，管腔内可见大量凋亡或坏死的生精细胞，occludin mRNA和蛋白表达水平明显降低。结论电磁脉冲辐射损伤小鼠睾丸BTB结构和功能，导致生精细胞凋亡或坏死。泌尿学80：225.e1-225.e6，2012年。(C)2012 Elsevier Inc.
OBJECTIVE To investigate the effects of electromagnetic pulse irradiation on the mouse blood-testicle barrier (BTB) and spermatogenesis.METHODS After whole body irradiation with 400 kV/m electromagnetic pulse irradiation, the mouse testicles and BTB permeability were observed using hematoxylin-eosin, Evans blue, and lanthanum nitrate as tracers. The expression of the BTB tight junction protein occludin was examined using real-time polymerase chain reaction and Western blotting.RESULTS At 1, 7, and 14 days after irradiation, the BTB structure was damaged, the BTB permeability was significantly increased, numerous apoptotic or necrotic spermatogenic cells were found in the lumen, and the mRNA and protein expression levels of occludin were markedly decreased. The BTB structure and occludin expression levels had gradually recovered by 21 and 28 days after irradiation.CONCLUSION Electromagnetic pulse irradiation damaged the structure and function of mouse BTB, resulting in apoptosis or necrosis of the spermatogenic cells. UROLOGY 80: 225.e1-225.e6, 2012. (C) 2012 Elsevier Inc.