Preparation of a photoaffinity probe for the vitamin D-dependent intestinal calcium binding protein: evidence for a calcium dependent, specific interaction with intestinal alkaline phosphatase.
Preparation of a photoaffinity probe for the vitamin D-dependent intestinal calcium binding protein: evidence for a calcium dependent, specific interaction with intestinal alkaline phosphatase.
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维生素 D 依赖性肠钙结合蛋白的光亲和探针的制备：钙依赖性、与肠碱性磷酸酶特异性相互作用的证据。
DOI：
10.1016/0006-291x(82)91854-x
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发表时间：
1982
影响因子：
3.1
通讯作者：
Leathers,V
中科院分区：
文献类型：
--
作者：
Norman,AW;Leathers,V
用4-叠氮基苯甲酰亚胺甲酯（MABI）与乳过氧化物酶-~（125）I-碘化的钙结合蛋白（CaBP）偶联，得到~（125）I-CaBP-MABI：[3 mol MABI/mol CaBP]，制备了维生素D依赖的鸡小肠钙结合蛋白（CaBP）光亲和探针。125 I-CaBP-MABI（28，000道尔顿）在模型系统中与牛肠碱性磷酸酶（AP）（67，000道尔顿）体外孵育后，发生UV光依赖性交联，产生分子量为95，000的缀合物（SDS-凝胶电泳）;与E.大肠杆菌碱性磷酸酶。发现125 I-CaBP-MABI-AP的形成仅在钙的存在下发生。
A photoaffinity probe for the vitamin D-dependent chick intestinal calcium binding protein (CaBP) has been prepared by conjugation of methyl-4-azidobenzoimidate (MABI) to lactoperoxidase-125 I-iodinated CaBP to yield 125 I-CaBP-MABI:[3 moles MABI per mole CaBP]. After incubation in vitro of 125 I-CaBP-MABI (28,000 daltons) in model systems with bovine intestinal alkaline phosphatase (AP)(67,000 daltons), a UV light-dependent crosslinking occurred to yield a conjugate with a molecular weight of 95,000 (by SDS-gel electrophoresis); no crosslinking occurred with E. coli alkaline phosphatase. The formation of the 125 I-CaBP-MABI-AP was found to occur only in the presence of calcium.