Cartilage Regeneration of Adipose-Derived Stem Cells in a Hybrid Scaffold From Fibrin-Modified PLGA
Cartilage Regeneration of Adipose-Derived Stem Cells in a Hybrid Scaffold From Fibrin-Modified PLGA
复制标题
纤维蛋白修饰 PLGA 混合支架中脂肪干细胞的软骨再生
DOI：
10.3727/096368909788341261
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Qi, Jin
中科院分区：
文献类型：
--
作者：
Wei, Yiyong;Hu, Hunyu;Qi, Jin
脂肪源性干细胞（ASCs）似乎是一种有用的干细胞群，已被证明具有多潜能。本研究的目的是评估ASCs在使用纤维蛋白修饰PLGA支架的混合支架的组织工程软骨中的应用。从兔脂肪组织中分离出ASCs。采用低温沉积技术制备PLGA支架，采用冷冻干燥法制备复合支架。体外将ASCs植入纤维蛋白修饰的PLGA支架上，与未修饰的PLGA支架相比，观察到细胞活力增强。蛋白多糖和胶原蛋白分析表明，纤维蛋白修饰支架成功诱导ASCs向体外软骨细胞分化。在体内进行了软骨再生的初步研究。组织学和免疫印迹观察表明，含有混合支架的ASCs对关节软骨缺损软骨再生的促进作用明显优于其他组。这些结果表明含有混合支架的ASCs是一种潜在的促进关节软骨再生的更有效的方法。
Adipose-derived stem cells (ASCs) appear to be a useful stem cell population, which has been shown to possess multipotentiality. The aim of this study was to evaluate the utility of ASCs in tissue-engineered cartilage using a hybrid scaffold from fibrin-modified PLGA scaffold. ASCs were isolated from rabbit adipose tissue. The PLGA scaffold was prepared by low-temperature deposition technology and the hybrid scaffold was fabricated by a freeze-drying method. When ASCs were seeded onto fibrin-modified PLGA scaffold in vitro, enhanced cellular viability was observed compared to unmodified PLGA scaffold. The analysis of proteoglycan and collagen If revealed that fibrin-modified scaffold Succeeded in inducing ASCs to differentiate into chondrocytes in vitro. A preliminary study on cartilage regeneration was also performed in vivo. Observation of histology and immunoblotting demonstrated that ASCs containing the hybrid scaffold promoted cartilage regeneration in the defects of articular cartilage much better than other groups. These results indicated that ASCs containing the hybrid scaffold are a more effective way to potentially enhance articular cartilage regeneration.