Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Healing of Standardized Bone Defects in the Alveolar Ridge: A Comparative Histomorphometric Study in Minipigs
Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Healing of Standardized Bone Defects in the Alveolar Ridge: A Comparative Histomorphometric Study in Minipigs
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DOI：
10.1016/j.joms.2008.06.036
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发表时间：
2009-02-01
影响因子：
1.9
通讯作者：
Marchetti, Claudio
中科院分区：
文献类型：
--
作者：
Pieri, Francesco;Lucarelli, Enrico;Marchetti, Claudio
目的：探讨骨髓间充质干细胞(MSCs)复合富血小板血浆(PRP)复合氟羟基磷灰石(FHA)支架修复小型猪下颌骨柱状缺损后骨再生的效果。材料与方法：8只成年小型猪双侧拔除双侧下颌前磨牙。术后2个月，每个根管形成直径3.5 mm、深度8 mm的标准缺损区4个。缺损处随机植入自体下颌骨、单纯FHA、PRP-FHA或MSCs-PRP-FHA。在缺损区放置可吸收的胶原膜，并缝合皮瓣。3个月后处死动物，并从缺损处采集活检标本进行组织学和组织形态计量学评估。结果：两种治疗方法都没有炎症或不良组织反应的证据。MSCs-PRP-FHA处理的部位在残留的移植颗粒之间显示出新的生机骨。PRP-FHA和FHA处理的部位在骨髓软组织背景中显示残留颗粒，并有中等数量的新骨形成。自体骨(46.97%)和MSCs-PRP-FHA(45.28%)产生的活力骨量明显高于PPP-FHA(37.95%)或单独使用FHA(36.03%)。此外，MSCs-PRP-FHA组移植颗粒与新骨的接触率显著高于PRP-FHA组和FHA组(分别为59.23%、48.37%和46.43%)。结论：在该动物模型中，在PRP-FHA中加入MSCs可促进3个月后的骨形成。(C)2009年美国口腔颌面外科医生协会
Purpose: The purpose of this study was to test the effect of the combination of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) incorporated into a fluorohydroxyapatite (FHA) scaffold on bone regeneration in cylindrical defects in the edentulous mandibular ridge of minipigs.Materials and Methods: Two mandibular premolar teeth were extracted bilaterally in 8 adult minipigs. After 2 months, 4 Standardized defects of 3.5 mm diameter and 8 mm depth were created in each root site. The defects were randomly grafted with autogenous mandibular bone, FHA alone, PRP-FHA, or MSCs-PRP-FHA. A resorbable collagen membrane was placed over the defect area and the flaps were sutured. The animals were sacrificed 3 months later and biopsy samples were taken from the defect sites for histologic and histomorphometric assessment.Results: There was no evidence of inflammation or adverse tissue reaction with either treatment. MSCs-PRP-FHA-treated sites showed new vital bone between residual grafting particles. PRP-FHA- and FHA-treated sites showed residual particles in a background of marrow soft tissue with a moderate quantity of newly formed bone. Autogenous bone (46.97%) and MSCs-PRP-FHA (45.28%) produced a significantly higher amount of vital bone than PPP-FHA (37.95%), or FHA alone (36.03%). Further, the MSCs-PRP-FHA-treated defects showed a significantly higher percentage of contact between graft particles and newly formed bone compared with PRP-FHA and FHA group (59.23% vs 48.37% and 46.43%, respectively).Conclusions: Our results Suggest that, in this animal model, the addition of MSCs to PRP-FHA enhances bone formation after 3 months. (C) 2009 American Association of Oral and Maxillofacial Surgeons