Effect of ovarian hormones on maturation of dendritic cells from peripheral blood monocytes in dogs.
Effect of ovarian hormones on maturation of dendritic cells from peripheral blood monocytes in dogs.
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DOI：
10.1292/jvms.14-0558
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Inaba T
中科院分区：
文献类型：
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作者：
Wijewardana V;Sugiura K;Wijesekera DP;Hatoya S;Nishimura T;Kanegi R;Ushigusa T;Inaba T
以前，我们曾报道卵巢激素影响抗大肠杆菌的免疫反应。从患子宫积脓的狗身上分离的大肠杆菌。为了研究免疫调节的机制，我们研究了卵巢激素对树突状细胞（DC），最有效的抗原呈递细胞的产生的影响。使用细胞因子混合物从外周血单核细胞（PBMO）分化DC。PBMO和未成熟DC均表达雌激素受体和孕激素受体。当向培养物中加入各种卵巢激素用于DC分化时，孕酮显著降低成熟DC上DC成熟标志物如CD 1a、CD 80和CD 86的表达。相反，向培养物中加入雌激素增加了CD86的表达，但没有增加其他成熟标记物的表达。此外，在孕酮存在下分化的DC不刺激PB中的同种异体单核细胞。综上所述，这些结果表明孕酮减少了DC的成熟，导致针对入侵病原体的免疫应答降低。
Previously, we reported that ovarian hormones affect the immune response against E. coli isolated from the dogs affected with pyometra. In order to investigate mechanisms underlying the immune modulation, we examined the effects of ovarian hormones on the generation of dendritic cells (DCs), the most potent antigen presenting cell. DCs were differentiated from peripheral blood monocytes (PBMOs) using a cytokine cocktail. Both estrogen receptor and progesterone receptors were expressed by the PBMOs and immature DCs. When various ovarian hormones were added to the culture for the DC differentiation, progesterone significantly decreased the expression of DC maturation markers, such as CD1a, CD80 and CD86, on mature DCs. Conversely, the addition of estrogen to the cultures increased the expression of CD86, but not other maturation makers. Furthermore, DCs differentiated in the presence of progesterone did not stimulate allogeneic mononuclear cells in PB. Taken together, these results indicate that progesterone diminishes the maturation of DCs, leading to decreased immune responses against invading pathogens.