Solution structure of DnaE intein from Nostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification
Solution structure of DnaE intein from Nostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification
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DOI：
10.1016/j.febslet.2009.03.058
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发表时间：
2009-05-06
期刊：
影响因子：
3.5
通讯作者：
Iwai, Hideo
中科院分区：
文献类型：
--
作者：
Oeemig, Jesper S.;Aranko, A. Sesilja;Iwai, Hideo
天然分裂的DnaE内含肽具有强大的蛋白质反式剪接活性和对剪接点处序列变异的高度耐受性。我们通过NMR光谱确定了NpuDnaE内含肽的单链变体的溶液结构。基于单链NpuDnaE内含肽的NMR结构和骨架动力学，我们设计了一个功能分裂的NpuDnaE内含肽的变体，其具有由六个残基组成的短C-末端半（C-内含肽）。在体内和体外蛋白质连接模型蛋白的新设计的分裂内含肽进行了演示。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.