Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress
Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress
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DOI：
10.4265/bio.27.169
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发表时间：
2022-01-01
影响因子：
1.2
通讯作者：
Tsuchido, Tetsuaki
中科院分区：
文献类型：
--
作者：
Asada, Ryoko;Sakamoto, Jin J.;Tsuchido, Tetsuaki
为了评估细菌孢子群体暴露于致死应力的损伤，我们提出了应用DiVSaL方法的理论基础，该方法已经被报道用于一般微生物作为双重继代培养方法。建立了一个数学模型，模型中考虑了对萌发系统和孢子体的损伤。在这一理论中，我们合理地假设，在随后的营养生长之前，活的和可萌发的孢子计数是恒定的，并且萌发和生长的延迟可以包括在“的”概念中。以前报道的生长无关性损伤。通过引入这些假设，可以认为双重继代培养方法也适用于孢子。作为该理论的应用实例，分析了热和紫外线照射处理的枯草芽孢杆菌孢子的生长延迟，并评估了受损伤孢子的数量。基于所获得的结果，热表明具有较高的伤害产生能力比紫外线照射。DiVSaL方法作为食品保存和卫生设计的工具的适用性。
To assess injury in bacterial spore populations exposed to lethal stress, we proposed a theoretical basis for applying the DiVSaL method, which has already been reported for general microorganisms as a double subculture method. We constructed a mathematical model in which both injuries to the germination system and the spore body were taken into the theory. In this theory, we reasonably assumed that the viable and germinable spore count is constant before the subsequent vegetative growth and that the delay of germination and outgrowth can be included in the concept of. injury previously reported as the growth-independent injury. By introducing these assumptions, the double subculture method can be considered to apply to spores as well. As examples of the application of this theory, the growth delays of Bacillus subtilis spores treated with heat and UV irradiation were analyzed and the numbers of injured spores were evaluated. Based on the results obtained, heat is indicated to have a higher injury generation ability than UV irradiation. The applicability of the DiVSaL method as a tool for food preservation and sanitation designs is presented.