The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans
The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans
复制标题
DOI：
10.1128/aem.00336-08
复制
发表时间：
2008-07-01
影响因子：
4.4
通讯作者：
Darby, Creg
中科院分区：
文献类型：
--
作者：
Drace, Kevin;Darby, Creg
嗜黄杆菌是一种昆虫病原体，也是线虫Steinernema carpocapsae的专性共生体。X.嗜热菌产生一种生物膜，附着在模式线虫秀丽隐杆线虫的头部，这是一种能力X。嗜热菌与鼠疫耶尔森氏菌和假结核耶尔森氏菌形成的生物膜相同。在耶尔森氏菌属中，嗜黄单胞菌生物膜需要4基因操纵子hmsHFRS。与耶尔森氏菌相似，X。嗜热菌生物膜被凝集素麦胚凝集素结合，表明P-连接的N-乙酰基-D-葡萄糖胺或N-乙酰基神经氨酸是细胞外基质的组分。具有不允许耶尔森氏菌生物膜附着的异常表面的秀丽线虫突变体也对嗜黄单胞菌生物膜具有抗性。一个不能在线虫上形成生物膜的嗜热假单胞菌hmsH突变体与S. carpocapsae，对昆虫天蛾的毒力也没有降低。
The bacterium Xenorhabdus nematophila is an insect pathogen and an obligate symbiont of the nematode Steinernema carpocapsae. X. nematophila makes a biofilm that adheres to the head of the model nematode Caenorhabditis elegans, a capability X. nematophila shares with the biofilms made by Yersinia pestis and Yersinia pseudotuberculosis. As in Yersinia spp., the X nematophila biofilm requires a 4-gene operon, hmsHFRS. Also like its Yersinia counterparts, the X. nematophila biofilm is bound by the lectin wheat germ agglutinin, suggesting that P-linked N-acetyl-D-glucosamine or N-acetylneuraminic acid is a component of the extracellular matrix. C elegans mutants with aberrant surfaces that do not permit Yersinia biofilm attachment also are resistant to X nematophila biofilms. An X nematophila hmsH mutant that failed to make biofilms on C elegans had no detectable defect in symbiotic association with S. carpocapsae, nor was virulence reduced against the insect Manduca sexta.