High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription
High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription
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DOI：
10.1093/emboj/cdg304
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发表时间：
2003-06-16
期刊：
影响因子：
11.4
通讯作者：
Wel, PA
中科院分区：
文献类型：
--
作者：
Gumbs, OH;Campbell, AM;Wel, PA
酵母Mot 1 p是Snf 2 p-ATP酶家族的一个丰富的保守成员，它在体外利用ATP的能量将TBP从DNA中解离出来，并在体内抑制基因转录，但矛盾的是，Mot 1 p功能的丧失也导致某些基因的转录减少。我们进行了实验，利用荧光标记的DNA，TBP，荧光各向异性光谱和天然凝胶电泳研究Mot 1 p行动。我们已经做了一些观察，最有趣的是，一个稳定的Mot 1 p-TBP复合物具有高亲和力结合TATA DNA的能力，尽管具有显着改变的特异性。我们提出，这种改变TBP-DNA识别是不可或缺的Mot 1 p的能力，调节转录，并进一步假设Mot 1 p-TBP复合物提供TBP TAF独立的mRNA编码基因。
Yeast Mot1p, an abundant conserved member of the Snf2p-ATPase family of proteins, both dissociates TBP from DNA in vitro using the energy of ATP and represses gene transcription in vivo, yet paradoxically, loss of Mot1p function also leads to decreased transcription of certain genes. We conducted experiments utilizing fluorescently labeled DNA, TBP, fluorescence anisotropy spectroscopy and native gel electrophoresis to study Mot1p action. We have made a number of observations, the most intriguing being that a stable Mot1p-TBP complex has the ability to bind TATA DNA with high affinity, albeit with dramatically altered specificity. We propose that this altered TBP-DNA recognition is integral to Mot1p's ability to regulate transcription, and further postulate that the Mot1p-TBP complex delivers TBP to TAF-independent mRNA encoding genes.