Multiple adenoviral serotypes demonstrate host range extension in the New Zealand rabbit ocular model.
Multiple adenoviral serotypes demonstrate host range extension in the New Zealand rabbit ocular model.
复制标题
多种腺病毒血清型在新西兰兔眼模型中证明了宿主范围的扩展。
DOI：
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发表时间：
1998
影响因子：
4.4
通讯作者：
Y. Gordon
中科院分区：
文献类型：
--
作者：
E. Romanowski;T. Araullo;Y. Gordon
目的
虽然几种人腺病毒血清型证明了在器官培养中在新西兰兔角膜中复制的遗传能力，但据报道，只有一种腺病毒（Ad）血清型Ad 5在新西兰兔眼中体内复制。本研究的目的是确定额外的腺病毒血清型是否可以将其宿主范围扩展到新西兰兔眼模型。
方法
角膜划痕后，每种病毒分离株在每只兔眼接种以下参考或临床腺病毒分离株之一：Ad 1 ATCC、Ad 1 Kmetz、Ad 2 ATCC、Ad 2 Wolf、Ad 5 ATCC、Ad 5麦克尤恩、Ad 6 ATCC、Ad 19 ATCC和Ad 8 Cray（5只兔），每只兔眼接种1.5 x 10（6）个空斑形成单位。在接种后第0、1、3、4、5、7、9、11、14、16、18和21天培养眼睛，并在A549细胞上测定其泪膜病毒滴度。
结果
Ad 19 ATCC和Ad 8 Cray显示没有明显的病毒复制。平均脱落时间分别为1.5和0.3天，Ad阳性眼的总百分比分别为13%和3%。与Ad 19 ATCC和Ad 8 Cray相反，所有其他分离株均表现出生产性感染。平均脱落时间为8 ~ 16天（P < 0.0001），总的Ad阳性眼百分率为33%~ 79%（P < 0.0002）。Ad 1 ATCC、Ad 1 Kmetz、Ad 2 ATCC、Ad 2 Wolf和Ad 6 ATCC的脱落持续时间与Ad 5麦克尤恩无统计学差异，而Ad 5 ATCC的脱落持续时间长于所有分离株（P < 0.0001）。
结论
这是在新西兰兔眼模型中首次证明1型、2型、5型和6型腺病毒的额外临床和参考分离株的宿主范围扩展。这些结果表明，宿主特异性不如以前认为的那么严格。
PURPOSE
Although several human adenoviral serotypes demonstrated the genetic capability of replicating in New Zealand rabbit corneas in organ culture, only a single adenovirus (Ad) serotype, Ad5, has been reported to replicate in vivo in New Zealand rabbit eyes. The purpose of this study was to determine whether additional adenoviral serotypes could extend their host range to the New Zealand rabbit ocular model.
METHODS
Six rabbits per viral isolate were inoculated in each eye after corneal scarification with 1.5 x 10(6) plaque-forming units per eye with one of the following reference or clinical adenovirus isolates: Ad1 ATCC, Ad1 Kmetz, Ad2 ATCC, Ad2 Wolf, Ad5 ATCC, Ad5 McEwen, Ad6 ATCC, Ad 19 ATCC, and Ad8 Cray (five rabbits). Eyes were cultured on days 0, 1, 3, 4, 5, 7, 9, 11, 14, 16, 18, and 21 after inoculation, and their tear film viral titers were determined on A549 cells.
RESULTS
Ad19 ATCC and Ad8 Cray demonstrated no apparent viral replication. The mean duration of shedding was 1.5 and 0.3 days, respectively, and the total percentage of Ad-positive eyes was 13% and 3%, respectively. In contrast to Ad19 ATCC and Ad8 Cray, all other isolates demonstrated productive infection. The mean duration of shedding was 8 to 16 days (P < 0.0001), and the total percentage of Ad-positive eyes was 33% to 79% (P < 0.0002). The durations of shedding for Ad1 ATCC, Ad1 Kmetz, Ad2 ATCC, Ad2 Wolf, and Ad6 ATCC did not differ statistically from Ad5 McEwen, whereas Ad5 ATCC demonstrated a duration of shedding longer than all isolates (P < 0.0001).
CONCLUSIONS
This was the first demonstration of host range extension by additional clinical and reference isolates of adenovirus types 1, 2, 5, and 6 in the New Zealand rabbit ocular model. These results suggested that host specificity was less stringent than previously thought.
局部 HPMPC 可抑制新西兰兔眼部复制模型中的 5 型腺病毒。
影响因子：
4.4
作者：
Gordon,YJ;Romanowski,EG;Araullo-Cruz,T
通讯作者：
Araullo-Cruz,T
新西兰兔 5 型腺病毒感染的眼部模型。
影响因子：
4.4
作者：
Gordon,YJ;Romanowski,E;Araullo-Cruz,T
通讯作者：
Araullo-Cruz,T