Serum levels of the proinflammatory cytokine interleukin-6 vary based on diagnoses in individuals with lumbar intervertebral disc diseases.
Serum levels of the proinflammatory cytokine interleukin-6 vary based on diagnoses in individuals with lumbar intervertebral disc diseases.
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DOI：
10.1186/s13075-015-0887-8
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发表时间：
2016-01-07
影响因子：
4.9
通讯作者：
Chahine NO
中科院分区：
文献类型：
--
作者：
Weber KT;Alipui DO;Sison CP;Bloom O;Quraishi S;Overby MC;Levine M;Chahine NO
许多椎间盘疾病引起腰痛（LBP）。促炎细胞因子和基质金属蛋白酶（MMPs）参与椎间盘病理。在这项研究中，我们检测了诊断为椎间盘突出（DH）、椎管狭窄（SS）或椎间盘退行性疾病（DDD）的人类受试者的血清细胞因子和MMPs水平与对照组的水平。将DH患者与其他诊断的患者（other Dx，按SS和DDD分组）进行比较，阐述基于循环细胞因子水平的病理机制。研究参与者从脊柱神经外科实践（n = 80）、背痛管理实践（n = 27）或对照队列（n = 26）中招募。治疗前采集血清样本，采用多重检测法检测白细胞介素(IL)-1β、IL-2、IL-4、IL-6、IL-8、IL-10、IL-12p70、IL-13、干扰素-γ、肿瘤坏死因子-α、MMP-1、MMP-3、MMP-9水平。通过诊断组和治疗组比较LBP患者和对照组的炎症和降解介质水平，控制性别、年龄和骨关节炎史的影响。Spearman相关系数用于检查年龄、体重指数（BMI）、症状持续时间和吸烟史之间的关系。与对照组相比，LBP患者血清IL-6水平显著升高。其他Dx引起的LBP患者的IL-6水平明显高于DH和对照组。LBP患者的血清MMP-1水平明显低于对照组，特别是DH患者。IL-6水平与BMI、症状持续时间和年龄呈正相关。MMP-1水平与年龄呈正相关。本临床研究的发现是首次检测DDD和SS患者循环细胞因子水平的结果，为IL-6在椎间盘疾病中更广泛的作用提供了证据，DDD或SS患者的血清细胞因子水平高于DH或对照组。这些发现表明腰痛患者有低度全身性炎症，循环细胞因子的生化分析可能有助于完善椎间盘疾病的个性化诊断。
Many intervertebral disc diseases cause low back pain (LBP). Proinflammatory cytokines and matrix metalloproteinases (MMPs) participate in disc pathology. In this study, we examined levels of serum cytokines and MMPs in human subjects with diagnoses of disc herniation (DH), spinal stenosis (SS), or degenerative disc disease (DDD) relative to levels in control subjects. Comparison between subjects with DH and those with other diagnoses (Other Dx, grouped from SS and DDD) was performed to elaborate a pathological mechanism based on circulating cytokine levels. Study participants were recruited from a spine neurosurgery practice (n = 80), a back pain management practice (n = 27), or a control cohort (n = 26). Serum samples were collected before treatment and were assayed by multiplex assays for levels of interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, interferon-γ, tumor necrosis factor-α, MMP-1, MMP-3, and MMP-9. Inflammatory and degradative mediator levels were compared for subjects with LBP and control subjects, by diagnosis and by treatment groups, controlling for effects of sex, age, and reported history of osteoarthritis. Spearman’s correlation coefficient was used to examine relationships with age, body mass index (BMI), symptom duration, and smoking history. Serum levels of IL-6 were significantly higher in subjects with LBP compared with control subjects. Participants with LBP due to Other Dx had significantly higher levels of IL-6 than DH and controls. Serum levels of MMP-1 were significantly lower in LBP subjects, specifically those with DH, than in control subjects. Positive correlations were found between IL-6 levels and BMI, symptom duration, and age. MMP-1 levels were positively correlated with age. The findings of the present clinical study are the results of the first examination of circulating cytokine levels in DDD and SS and provide evidence for a more extensive role of IL-6 in disc diseases, where patients with DDD or SS have higher serum cytokine levels than those with DH or control subjects. These findings suggest that LBP subjects have low-grade systemic inflammation, and biochemical profiling of circulating cytokines may assist in refining personalized diagnoses of disc diseases.