THE ROLE OF CELL-ADHESION MOLECULES IN NEURITE OUTGROWTH ON MULLER CELLS
THE ROLE OF CELL-ADHESION MOLECULES IN NEURITE OUTGROWTH ON MULLER CELLS
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DOI：
10.1016/0012-1606(90)90178-l
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发表时间：
1990-03-01
影响因子：
2.7
通讯作者：
LEMMON, V
中科院分区：
文献类型：
--
作者：
DRAZBA, J;LEMMON, V
研究了神经细胞粘附分子 (NCAM)、L1、N-钙粘蛋白和整合素在各种基质上神经突生长中的作用。将针对这些细胞表面分子的抗体添加到小鸡视网膜的外植体中，并检查来自视网膜神经节细胞的神经突，以了解抗体对神经突长度和束状运动的影响。在层粘连蛋白上，抗整合素抗体完全抑制神经突生长。相同的抗体不抑制多聚赖氨酸或穆勒细胞的神经突生长。 NCAM、L1 和 N-钙粘蛋白的抗体不会显着抑制层粘连蛋白上的神经突生长，但对 Mueller 细胞产生显着的抑制。抗 L1 抗体对神经胶质细胞上神经突生长的抑制支持了 L1 能够以异嗜性结合机制发挥作用的假设。对于层粘连蛋白，抗 N-钙粘蛋白和抗 L1 均会导致视网膜神经节细胞的神经突解束，而抗 NCAM 则不会。这些抗体均未对 Mueller 细胞产生解聚作用。结果表明，当轴突从视网膜生长到顶盖时，这三种细胞粘附分子在与神经胶质细胞的相互作用中可能非常重要，而在沿该途径的轴突-轴突相互作用中可能不太重要。没有发现证据支持整合素在穆勒细胞轴突生长中的作用。
The roles of neural cell adhesion molecule (NCAM), L1, N-cadherin, and integrin in neurite outgrowth on various substrates were studied. Antibodies against these cell surface molecules were added to explants of chick retina and the neurites from retinal ganglion cells were examined for effects of the antibodies on neurite length and fasciculation. On laminin, an anti-integrin antibody completely inhibited neurite outgrowth. The same antibody did not inhibit neurite outgrowth of polylysine or Mueller cells. Antibodies to NCAM, L1, and N-cadherin did not significantly inhibit neurite outgrowth on laminin but produced significant inhibition on Mueller cells. The inhibition of neurite outgrowth on glia by anti-L1 antibodies supports the hypothesis that L1 is capable of acting in a heterophilic binding mechanism. On laminin, both anti-N-cadherin and anti-L1 caused defasciculation of neurites from retinal ganglion cells, while anti-NCAM did not. None of these antibodies produced defasciculation on Mueller cells. The results indicate that these three cell adhesion molecules may be very important in interactions with glia as axons grow from the retina to the tectum and may be less important in axon-axon interactions along this pathway. No evidence was found supporting the role of integrins in axon growth on Mueller cells.