Interspecies interactions stimulate diversification of the Streptomyces coelicolor secreted metabolome.
Interspecies interactions stimulate diversification of the Streptomyces coelicolor secreted metabolome.
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DOI：
10.1128/mbio.00459-13
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发表时间：
2013-08-20
期刊：
影响因子：
6.4
通讯作者：
Kolter R
中科院分区：
文献类型：
--
作者：
Traxler MF;Watrous JD;Alexandrov T;Dorrestein PC;Kolter R
土壤中有各种各样的微生物群落，包括丝状放线菌(放线菌)。这些细菌一直是有用代谢物的丰富来源，包括抗菌剂、抗真菌药、抗癌剂、铁载体和免疫抑制剂。虽然人类长期以来一直将这些化合物用于治疗目的，但这些天然产物在调节放线菌之间的相互作用中可能发挥的作用一直很难确定。作为在系统水平上理解这些化学相互作用的第一步，我们使用了新兴的纳米解吸电喷雾电离(NanoDESI)和基质辅助激光解吸电离飞行时间(MALDI-TOF)成像质谱学技术，获得了天蓝色链霉菌与其他五种放线菌相互作用的全球化学视图。在每个相互作用中，与天蓝色链球菌菌落相关的大多数分泌化合物都是独一无二的，表明天蓝色链球菌对此有特殊的反应。光谱网络揭示了天蓝色链霉菌在几次相互作用中产生的一系列未知化合物。这些化合物组成了至少12种不同的去铁胺的延伸系列，这些去铁胺具有不同长度的酰基侧链；它们的产生是由邻近菌株产生的铁载体引发的。综上所述，这些结果表明放线菌之间的化学相互作用表现出高度的复杂性和特异性，并可以驱动不同的次生代谢物的产生。放线菌，土壤中的丝状放线菌，是有用的药物化合物的最深自然来源，包括抗生素、抗真菌药物和抗癌药物。人们对开发新的策略以增加实验室中放线菌分泌的代谢物的多样性非常感兴趣。在这里，我们使用了几种代谢组学方法来检查这些细菌在成对共培养时产生的化学物质。我们发现，这些物种间的相互作用刺激了许多化合物的产生，而这些化合物不是在它们单独生长时产生的。在这些化合物中，有至少12种不同的名为去铁胺的分子，去铁胺是细菌用来收集铁的一种铁载体。还观察到了许多其他未知身份的化合物，并且化合物的产生模式在相互作用集之间差别很大。这些发现表明，放线菌之间的化学相互作用令人惊讶地复杂，共培养可能是从放线菌中寻找新分子的一种有前途的策略。
Soils host diverse microbial communities that include filamentous actinobacteria (actinomycetes). These bacteria have been a rich source of useful metabolites, including antimicrobials, antifungals, anticancer agents, siderophores, and immunosuppressants. While humans have long exploited these compounds for therapeutic purposes, the role these natural products may play in mediating interactions between actinomycetes has been difficult to ascertain. As an initial step toward understanding these chemical interactions at a systems level, we employed the emerging techniques of nanospray desorption electrospray ionization (NanoDESI) and matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) imaging mass spectrometry to gain a global chemical view of the model bacterium Streptomyces coelicolor interacting with five other actinomycetes. In each interaction, the majority of secreted compounds associated with S. coelicolor colonies were unique, suggesting an idiosyncratic response from S. coelicolor. Spectral networking revealed a family of unknown compounds produced by S. coelicolor during several interactions. These compounds constitute an extended suite of at least 12 different desferrioxamines with acyl side chains of various lengths; their production was triggered by siderophores made by neighboring strains. Taken together, these results illustrate that chemical interactions between actinomycete bacteria exhibit high complexity and specificity and can drive differential secondary metabolite production. Actinomycetes, filamentous actinobacteria from the soil, are the deepest natural source of useful medicinal compounds, including antibiotics, antifungals, and anticancer agents. There is great interest in developing new strategies that increase the diversity of metabolites secreted by actinomycetes in the laboratory. Here we used several metabolomic approaches to examine the chemicals made by these bacteria when grown in pairwise coculture. We found that these interspecies interactions stimulated production of numerous chemical compounds that were not made when they grew alone. Among these compounds were at least 12 different versions of a molecule called desferrioxamine, a siderophore used by the bacteria to gather iron. Many other compounds of unknown identity were also observed, and the pattern of compound production varied greatly among the interaction sets. These findings suggest that chemical interactions between actinomycetes are surprisingly complex and that coculture may be a promising strategy for finding new molecules from actinomycetes.