Sex-related differences in platelet aggregation: influence of the hematocrit.
Sex-related differences in platelet aggregation: influence of the hematocrit.
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血小板聚集的性别相关差异：血细胞比容的影响。
DOI：
10.1182/blood.v56.1.38.bloodjournal56138
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发表时间：
1980
期刊：
影响因子：
20.3
通讯作者：
J. Hirsh
中科院分区：
文献类型：
--
作者：
J. Kelton;P. Powers;J. Julian;V. Boland;C. Carter;M. Gent;J. Hirsh
一些研究人员最近报告说，与年龄匹配的男性血小板相比，人类女性血小板在体外具有更高的反应性。对这些观察结果的一个可能的解释是，女性较低的红细胞压积导致抗凝剂的分布体积更大，从而导致更高的电离钙浓度。来检验这一可能性。我们对11名男性和11名女性健康受试者的血小板进行了二磷酸腺苷(ADP)和胶原聚集。当使用3.8%柠檬酸钠作为抗凝剂(9：1)对血液样本进行聚集时。V：V.血液：抗凝剂)。在广泛的ADP和胶原浓度范围内，女性血小板的反应性明显高于男性血小板(p<；0.01)。然后，对相同的受试者进行了重新测试。但在这种情况下，根据数学推导的标准曲线调整了红细胞压积的柠檬酸盐浓度。以前提到的女性血小板反应性增加的现象消失了，男性和女性血小板对ADP或胶原的反应性没有显著差异。已报道的体外血小板反应性的性别差异是由柠檬酸盐分布隔间的大小不同引起的人工制品造成的。据报道，在某些疾病状态下，类似的现象可能是导致血小板反应性增加的原因。经常伴随伴随的贫血。
A number of investigators have recently reported that human female platelets have increased reactivity in vitro compared to platelets from age-matched males. A possible explanation for these observations is that the lower hematocrit in females results in a larger volume of distribution of the anticoagulant, with resultant higher ionized calcium concentrations. To examine this possibility. we performed adenosine diphosphate (ADP) and collagen aggregations on platelets obtained from 1 1 male and 1 1 female healthy subjects. When aggregations were performed on blood samples using the standard amount of 3.8% sodium citrate as an anticoagulant (9:1 . v:v. blood:anticoagulant). the female platelets were significantly more reactive than the male platelets over a wide range of ADP and collagen concentrations (p < 0.01 ). The same subjects were then retested. but on this occasion the citrate concentration was adjusted for the hematocrit according to a mathematically derived standard curve. The previously noted increased reactivity of female platelets disappeared, and there was no significant difference in reactivity to either ADP or collagen between the male and female platelets. The reported in vitro sex differences in platelet reactivity is contributed to by an artifact caused by differences in the size of the citrate distribution compartment. It is possible that a similar phenomenon is responsible for the increased platelet reactivity reported in certain disease states. which frequently have a concomitantly associated anemia.