Enhancement of the transfer of alpha-tocopherol between liposomes and mitochondria by rat-liver protein(s).
Enhancement of the transfer of alpha-tocopherol between liposomes and mitochondria by rat-liver protein(s).
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通过大鼠肝脏蛋白增强α-生育酚在脂质体和线粒体之间的转移。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
S. Nojima
中科院分区：
文献类型：
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作者：
H. Mowri;Y. Nakagawa;K. Inoue;S. Nojima
大鼠肝脏中的一种胞浆蛋白促进了α-生育酚从脂质体到线粒体的转移。该蛋白是从磷脂酰胆碱特异的交换蛋白中分离出来的。这种刺激是对α-生育酚的专一性的，并且生育酚对胞浆蛋白的亲和力顺序为：α-生育酚大于γ-生育酚远大于α-生育酚醋酸酯和α-生育酚醌。转移刺激蛋白可以作为载体，因为当含有α-生育酚的脂质体与胞浆孵育时，可以检测到中间体(α-生育酚)-蛋白质复合体。这种转移刺激活性不仅存在于肝脏，也存在于心、脾和肺中。
alpha-Tocopherol transfer from liposomes to mitochondria was enhanced by a cytosolic protein in rat liver. This protein was separated from the exchange protein specific for phosphatidylcholine. The stimulation was specific for alpha-tocopherol and the "affinity" order of tocopherols for the cytosolic protein was found to be as follows: alpha-tocopherol greater than gamma-tocopherol much greater than alpha-tocopheryl acetate, alpha-tocopherol quinone. The transfer-stimulating protein may act as a carrier, since an intermediate (alpha-tocopherol)-protein complex could be detected when liposomes containing alpha-tocopherol were incubated with the cytosol. This transfer-stimulating activity was present not only in liver, but also in heart, spleen and lung.