Label-free detection of multiple bacterial pathogens using light-scattering sensor
Label-free detection of multiple bacterial pathogens using light-scattering sensor
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DOI：
10.1016/j.bios.2008.08.053
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发表时间：
2009-02-15
影响因子：
12.6
通讯作者：
Bhunia, Arun K.
中科院分区：
文献类型：
--
作者：
Banada, Padmapriya P.;Huff, Karleigh;Bhunia, Arun K.
快速检测和分类细菌病原体的技术对确保粮食供应至关重要。本报告描述了一种光散射传感器，能够实时检测和识别多种病原体的菌落，而不需要标记试剂或生化处理。由单个亲本细胞的后代组成的细菌菌落在635 nm处散射光，产生独特的前向散射特征。Zernike矩不变量和Haralick描述符有助于特征提取和散点签名图像库的构建。该方法能够在属和种水平上区分李斯特菌、葡萄球菌、沙门氏菌、弧菌和埃希氏菌的细菌培养，对于来自食物或实验感染动物的样品，准确率为90-99%。细菌产生的不同数量的外多糖引起相位调制分布的变化，导致明显不同的散射特征。在一个强大的数据库的帮助下，该方法可以潜在地检测和识别任何细菌菌落。与其他方法不同的是，它不破坏样本，但保留完整样本，以便在需要时为法医或疫情调查进行其他确认性检测。(C) 2008 Elsevier B.V.版权所有
Technologies for rapid detection and classification of bacterial pathogens are crucial for securing the food supply. This report describes a light-scattering sensor capable of real-time detection and identification of colonies of multiple pathogens without the need for a labeling reagent or biochemical processing. Bacterial colonies consisting of the progeny of a single parent cell scatter light at 635 nm to produce unique forward-scatter signatures. Zernike moment invariants and Haralick descriptors aid in feature extraction and construction of the scatter-signature image library. The method is able to distinguish bacterial cultures at the genus and species level for Listeria, Staphylococcus, Salmonella, Vibrio, and Escherichia with an accuracy of 90-99% for samples derived from food or experimentally infected animal. Varied amounts of exopolysaccharide produced by the bacteria causes changes in phase modulation distributions, resulting in strikingly different scatter signatures. With the aid of a robust database the method can potentially detect and identify any bacteria colony essentially instantaneously. Unlike other methods, it does not destroy the sample, but leaves it intact for other confirmatory testing, if needed, for forensic or outbreak investigations. (C) 2008 Elsevier B.V. All rights reserved.