INDUCTION OF SQUAMOUS DIFFERENTIATION OF NORMAL HUMAN BRONCHIAL EPITHELIAL-CELLS BY SMALL AMOUNTS OF SERUM
INDUCTION OF SQUAMOUS DIFFERENTIATION OF NORMAL HUMAN BRONCHIAL EPITHELIAL-CELLS BY SMALL AMOUNTS OF SERUM
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DOI：
10.1111/j.1432-0436.1984.tb01361.x
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
SHAMSUDDIN, AM
中科院分区：
文献类型：
--
作者：
LECHNER, JF;HAUGEN, A;SHAMSUDDIN, AM
低Ca（110 .亩）。M)无血清培养基（LHC-1）克隆生长正常人支气管上皮细胞（NHBE）。菌落内的NHBE细胞很小（平均表面积= 1250 .mu）。2)，很少迁移，张力丝很少，繁殖平均种群倍增时间为28 h。在LHC-1培养基中添加少量血源性血清（少至2%）可显著降低克隆生长速度。血清的生长抑制作用是由于诱导鳞状（终末）分化。血清能迅速增大细胞的大小（平均表面积= 4900 .mu.2）。细胞获得大量的桥粒连接和广泛的角蛋白束网络。人肺癌细胞在含有高达8%血清的LHC-1培养基中以克隆密度快速繁殖。虽然已知培养基中高浓度的钙离子会在没有血清的情况下诱导表皮角质形成细胞的鳞状分化，但高水平的钙离子（高达1000 .mu）可以诱导表皮角质形成细胞的鳞状分化。M)增加了桥粒体连接的数量，但对NHBE细胞的克隆生长速度和大小没有显著影响。高浓度钙（超过450 .亩）。M)被发现能增强血清诱导分化的活性。霍乱毒素（10 ng/ml）抑制血清的诱导分化活性。血清可以诱导NHBE细胞的鳞状分化，并且这些血清因子的效力可以调节。肺癌细胞与正常细胞的不同之处在于在血清的存在下不发生分化。
A low Ca (110 .mu.M) serum-free medium (LHC-1) was reported for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1250 .mu.2), rarely migratory, have few tonofilaments and multiply with an average population doubling time of 28 h. Adding small amounts of blood-derived serum to LHC-1 medium (as little as 2%) significantly decreased the clonal growth rate. The growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4900 .mu.2). The cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. Human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8% serum. Although high concentrations of Ca ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2+ (up to 1000 .mu.M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. High concentrations of Ca (above 450 .mu.M) were found to potentiate serum differentiation-inducing activity. Cholera toxin (10 ng/ml) inhibited the differentiation-inducing activity of serum. Squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. Lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.