Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II.
Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II.
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DOI：
10.1083/jcb.201005134
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发表时间：
2010-12-27
期刊：
影响因子：
--
通讯作者：
Bi E
中科院分区：
文献类型：
--
作者：
Fang X;Luo J;Nishihama R;Wloka C;Dravis C;Travaglia M;Iwase M;Vallen EA;Bi E
酵母肌球蛋白II的尾部通过两种不同的分子途径定位于分裂位点，足以促进肌动球蛋白环组装、沟内陷和指导ECM重塑。在动物和真菌细胞中的胞质分裂利用收缩性肌动球蛋白环（AMR）。然而，肌球蛋白II是如何靶向分裂位点并促进AMR组装，以及AMR如何在胞质分裂期间与膜运输协调，仍然知之甚少。在这里，我们表明，Myo1是一个双头的肌球蛋白II在酿酒酵母，Myo1本地化的分裂位点通过两个不同的靶向信号在其尾部，在细胞周期中依次作用。在胞质分裂之前，Myo 1的定位取决于septin结合蛋白Bni 5。在胞质分裂期间，Myo1定位依赖于IQGAP Iqg1。我们还表明，Myo1尾巴是足以促进组装的“无头”AMR，引导膜沉积和细胞外基质重塑的分裂网站。我们的研究建立了肌球蛋白II的双相靶向机制，并强调了AMR在胞质分裂中的作用。
The tail of yeast myosin II is localized to the division site by two distinct molecular pathways and sufficient for promoting actomyosin ring assembly, furrow ingression, and guidance in ECM remodeling. Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with membrane trafficking during cytokinesis, remains poorly understood. Here we show that Myo1 is a two-headed myosin II in Saccharomyces cerevisiae, and that Myo1 localizes to the division site via two distinct targeting signals in its tail that act sequentially during the cell cycle. Before cytokinesis, Myo1 localization depends on the septin-binding protein Bni5. During cytokinesis, Myo1 localization depends on the IQGAP Iqg1. We also show that the Myo1 tail is sufficient for promoting the assembly of a “headless” AMR, which guides membrane deposition and extracellular matrix remodeling at the division site. Our study establishes a biphasic targeting mechanism for myosin II and highlights an underappreciated role of the AMR in cytokinesis beyond force generation.
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