Salp25D, an Ixodes scapularis antioxidant, is 1 of 14 immunodominant antigens in engorged tick salivary glands
Salp25D, an Ixodes scapularis antioxidant, is 1 of 14 immunodominant antigens in engorged tick salivary glands
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DOI：
10.1086/323351
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发表时间：
2001-10-15
影响因子：
6.4
通讯作者：
Fikrig, E
中科院分区：
文献类型：
--
作者：
Das, S;Banerjee, G;Fikrig, E
感染肩突硬蜱的家兔或豚鼠获得对蜱叮咬的抵抗力，这种现象称为蜱免疫，部分由抗体介导。为了确定在宿主中引发抗体的唾液腺抗原，用I.以蜱免疫兔血清为探针，对肩胛肌唾液腺cDNA表达文库进行检测。致敏兔的血清在抗体筛选试验中强烈识别100，000个文库克隆中的47个。这47个克隆编码14种不同的I.肩胛肌基因，包括谷胱甘肽过氧化物酶同源物。这14个基因的表达，在充血蜱唾液腺证实了逆转录聚合酶链反应。重症监护肩胛谷胱甘肽过氧化物酶同源物salp25D在未进食和进食的若虫唾液腺中表达。重组Salp25D能够在还原型谷胱甘肽和谷胱甘肽还原酶的存在下催化过氧化氢的还原。这些结果将I.肩胛肌，并证明蜱唾液中存在有效的抗氧化剂。
Rabbits or guinea pigs infested with Ixodes scapularis acquire resistance to tick bites, a phenomenon, known as tick immunity, that is partially mediated by antibody. To determine the salivary gland antigens that elicit antibodies in the host, an I. scapularis salivary gland cDNA expression library was probed with serum from tick-immune rabbits. Sera from sensitized rabbits strongly recognized 47 of 100,000 library clones in an antibody-screening assay. These 47 clones encoded 14 different I. scapularis genes, including a glutathione peroxidase homologue. Expression of these 14 genes in engorged tick salivary glands was confirmed by reverse-transcription polymerase chain reaction. The I. scapularis glutathione peroxidase homologue, named salp25D, was expressed in both unfed and fed nymphal salivary glands. Recombinant Salp25D was able to catalyze the reduction of hydrogen peroxide in the presence of reduced glutathione and glutathione reductase. These results categorize the prominent salivary gland proteins in I. scapularis and demonstrate the presence of a potent antioxidant in tick saliva.