Down-regulation of miRNA-221 triggers osteogenic differentiation in human stem cells
Down-regulation of miRNA-221 triggers osteogenic differentiation in human stem cells
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DOI：
10.1007/s10529-012-0934-3
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发表时间：
2012-08-01
影响因子：
2.7
通讯作者：
Soleimani, Masoud
中科院分区：
文献类型：
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作者：
Bakhshandeh, Behnaz;Hafizi, Maryam;Soleimani, Masoud
尽管计算机证据很有趣，但 mir-221 在成骨中的具体作用尚未研究。我们评估了瞬时转染的抗 mir-221 在人非限制性成体干细胞和人间充质干细胞中的转录和翻译方面的成骨诱导作用。在转染的非限制性成体干细胞中，一些成骨标记物的转录是对照的两倍，骨桥蛋白和骨钙素的翻译分别从9%增加到39%和从0%增加到21%。转染的间充质干细胞中转录的成骨标志物的上调比对照高 50 倍，但没有观察到翻译的显着变化。在这些分析之前，验证了干细胞的真实性、其成骨分化和转染效率。因此，mir-221 的瞬时调节表明了一种快速诱导成骨的机制，作为基于细胞的治疗的有用策略。
Despite interesting in silico evidence, the specific role of mir-221 in osteogenesis has not been studied. We evaluated the osteogenic induction of transient-transfected anti-mir-221 in human unrestricted somatic stem cells and human mesenchymal stem cells both transcriptionally and translationally. In transfected unrestricted somatic stem cells, transcriptions of some osteogenic markers were twice that of the control and translations of osteopontin and osteocalcin were increased from 9 to 39 % and from 0 to 21 %, respectively. Up-regulation of transcribed osteogenic markers in transfected mesenchymal stem cells were 50 times greater than controls while no significant change in translations were observed. Prior to these analyses, the authenticity of stem cells, their osteogenic differentiation and transfection efficiency were verified. Transient modulation of mir-221 therefore suggests a mechanism for rapid induction of osteogenesis as a useful strategy for cell-based therapy.