PRIMARY STRUCTURE OF GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN FROM BOVINE BONE
PRIMARY STRUCTURE OF GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN FROM BOVINE BONE
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DOI：
10.1073/pnas.73.10.3374
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
RAMAN, N
中科院分区：
文献类型：
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作者：
PRICE, PA;POSER, JW;RAMAN, N
给出了牛骨中含有γ-羧基谷氨酸的蛋白质的氨基酸序列。49个残基中的43个的序列是在完整的蛋白质和胰蛋白酶多肽上自动确定的。其余的用常规的胰酶和胰凝乳酶多肽测定。序列中的第9个残基被鉴定为4-羟基脯氨酸。该蛋白质含有3个γ-羧基谷氨酸残基，位于第17、21和24位。讨论了这些不寻常的氨基酸在蛋白质与羟基磷灰石晶体结合作用中的作用。
The amino-acid sequence of the .gamma.-carboxyglutamic acid-containing protein of bovine bone is presented. The sequence of 43 of the 49 residues was determined automatically on the intact protein and on a tryptic peptide. The remainder was determined by conventional procedures on tryptic and chymotryptic peptides. Residue 9 in the sequence was identified as 4-hydroxyproline. The protein contains 3 .gamma.-carboxyglutamic acid residues, which are located at positions 17, 21 and 24. The role of these unusual amino acids in the binding interaction between the protein and hydroxyapatite crystals is discussed.