The natural and anthropogenic microbiota of Glerá, a sub-arctic river in northeastern Iceland
The natural and anthropogenic microbiota of Glerá, a sub-arctic river in northeastern Iceland
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DOI：
10.1016/j.ibiod.2012.04.001
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发表时间：
2013-10
影响因子：
4.8
通讯作者：
María Markúsdóttir;S. Heiðmarsson;Arnheiður Eyþórsdóttir;K. Magnússon;O. Vilhelmsson
中科院分区：
文献类型：
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作者：
María Markúsdóttir;S. Heiðmarsson;Arnheiður Eyþórsdóttir;K. Magnússon;O. Vilhelmsson
冰岛北部的gler<e:1>河为评估亚北极河流微生物群的人为影响提供了一个方便的模型。这条河流发源于一个原始的山谷，流经一个垃圾填埋场，在那里渗滤液流入河流，随后流经一个小城市地区。我们的目的是表征河流微生物群在其原始状态和人为影响状态下的特征，特别关注潜在的表面活性剂降解剂，其在河水中的活性可能有助于河流的自净化能力。我们从河流的原始源头到河口的10个采样点对河水和河床沉积物进行了采样，以培养细菌和提取DNA。还对集水区的其他一些生物群落进行了采样。对170株菌株进行了纯化和活菌培养，并进行了部分分析，以进行16S rRNA基因测序鉴定，并对选定的表面活性剂、聚糖、蛋白质和甘油三酯进行了生物降解。蛋白质降解假单胞菌在培养收集中有很强的代表性，其中许多也可以降解选定的表面活性剂。该集合还包括γ变形菌门的其他成员以及α变形菌门、β变形菌门、杆菌门、放线菌门、黄杆菌门和鞘菌。除Betaproteobacteria外，所有类别的几个分离株都显示出生物降解活性。
The river Glerá in Northern-Iceland presents a convenient model for the evaluation of anthropogenic effects on sub-Arctic river microbiota. The river originates in a pristine valley, flows past a landfill site where leachate merges into the river, and subsequently through a small urban area. Our aim was to characterize the river microbiota in both its pristine and anthropogenically impacted state, particularly focusing on prospective surfactant degraders whose activity in the river water might contribute to the river's self-purification capacity. We sampled river water and riverbed sediment at 10 sampling sites that spanned the river from its pristine source to the estuary for culturable bacteria and extractable DNA. A few other biotopes in the catchment area were also sampled. A confirmed pure and viable culture collection of 170 isolates was generated and partially analyzed for identification by 16S rRNA gene sequencing, and for biodegradation of selected surfactants as well as glycans, protein and triglyceride. Protein-degrading pseudomonads are strongly represented in the culture collection, many of whom can also degrade selected surfactants. The collection also contains other members of the Gammaproteobacteria as well as the Alphaproteobacteria, Betaproteobacteria, Bacilli, Actinobacteria, Flavobacteria, and Sphingobacteria. Several isolates of all classes, except the Betaproteobacteria, displayed biodegradative activity.