Dendritic morphology, local circuitry, and intrinsic electrophysiology of principal neurons in the entorhinal cortex of macaque monkeys
Dendritic morphology, local circuitry, and intrinsic electrophysiology of principal neurons in the entorhinal cortex of macaque monkeys
复制标题
DOI：
10.1002/cne.20014
复制
发表时间：
2004-03-08
影响因子：
2.5
通讯作者：
Amaral, DG
中科院分区：
文献类型：
--
作者：
Buckmaster, PS;Alonso, A;Amaral, DG
人们对灵长类动物内嗅皮层单个神经元的神经解剖学或电生理学特性知之甚少。我们在猕猴的内嗅切片制备中使用细胞内记录和生物胞素标记技术来研究主要神经元的形态和内在电生理学。这些神经元此前已在大鼠身上进行了最广泛的研究。在猴子中，第 11 层神经元通常是星状细胞，就像在大鼠中一样，但它们偶尔也呈金字塔形。它们倾向于释放一系列动作电位，而不是爆发动作电位，并且有些表现出阈下膜电位振荡。第三层神经元是锥体状的，它们似乎不表现出膜电位振荡。树突和轴突侧支的分布表明第 11 层和第 III 层的神经元通过关联纤维网络相互连接。第五层和第六层神经元呈锥体状，倾向于释放动作电位序列。树突和轴突侧枝的分布表明，V 层和 VI 层存在主要神经元的关联网络，并且它们还将轴突侧枝投射到浅层。重要的是，猴子的内嗅皮质神经元似乎与大鼠的内嗅皮质神经元表现出显着差异。从形态上看，猴子内嗅层 11 和 III 的神经元比大鼠具有更多的初级树突、更多的树突分支和更长的树突总长度。从电生理学角度来看，猴子的第二层神经元表现出较少的下垂，并且阈下振荡不太稳健且较慢。一些猴子第三层神经元会释放大鼠中没有的动作电位爆发。这项研究揭示的种间差异可能会影响灵长类动物内嗅皮层的信息处理和病理生理过程。 (C) 2004 Wiley-Liss, Inc.
Little is known about the neuroanatomical or electrophysiological properties of individual neurons in the primate entorhinal cortex. We have used intracellular recording and biocytin-labeling techniques in the entorhinal slice preparation from macaque monkeys to investigate the morphology and intrinsic electrophysiology of principal neurons. These neurons have previously been studied most extensively in rats. In monkeys, layer 11 neurons are usually stellate cells, as in rats, but they occasionally have a pyramidal shape. They tend to discharge trains, not bursts, of action potentials, and some display subthreshold membrane potential oscillations. Layer III neurons are pyramidal, and they do not appear to display membrane potential oscillations. The distribution of dendrites and of axon collaterals suggests that neurons in layers 11 and III are interconnected by a network of associational fibers. Layer V and VI neurons are pyramidal and tend to discharge trains of action potentials. The distribution of dendrites and axon collaterals suggests that there is an associative network of principal neurons in layers V and VI, and they also project axon collaterals toward superficial layers. Importantly, entorhinal cortical neurons in monkeys appear to exhibit significant differences from those in rats. Morphologically, neurons in monkey entorhinal layers 11 and III have more primary dendrites, more dendritic branches, and greater total dendritic length than in rats. Electrophysiologically, layer II neurons in monkeys exhibit less sag, and subthreshold oscillations are less robust and slower. Some monkey layer III neurons discharge bursts of action potentials that are not found in rats. The interspecies differences revealed by this study may influence information processing and pathophysiological processes in the primate entorhinal cortex. (C) 2004 Wiley-Liss, Inc.