FAST AXONAL-TRANSPORT IN SQUID GIANT-AXON
FAST AXONAL-TRANSPORT IN SQUID GIANT-AXON
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DOI：
10.1126/science.6183744
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
GILBERT, SP
中科院分区：
文献类型：
--
作者：
ALLEN, RD;METUZALS, J;GILBERT, SP
视频增强对比差分干涉对比显微镜揭示了鱿鱼巨大轴突轴突运输的新特征，在此之前，传统显微镜没有检测到任何运动。新发现的主要特征是大量的亚微观粒子，可能是30-50纳米的囊泡和其他管状囊泡元素，平行于线性元素移动，主要是在正方向，也在逆行方向，以稳定的速度范围达到。+-。5 .mu.m / s。培养基（0.2 ~ 0.6 μ）。M)和大(0.8亩。M)粒子运动得更慢，更断断续续，有时倾向于表现出弹性反冲。小颗粒和大颗粒在实际转运过程中的行为表明，轴突运输中细胞器运动的基本过程不是跳跃式的，而是连续的。
Video-enhanced contrast-differential interference contrast microscopy has revealed new features of axonal transport in the giant axon of the squid, where no movement had been detected previously by conventional microscopy. The newly discovered dominant feature is vast numbers of submicroscopic particles, probably 30-50 nm vesicles and other tubulovesicular elements, moving parallel to linear elements, primarily in the orthograde direction but also in a retrograde direction, at a range of steady velocities up to .+-. 5 .mu.m/s. Medium (0.2 to 0.6 .mu.m) and large (0.8 .mu.m) particles move more slowly and more intermittently with a tendency at times to exhibit elastic recoil. The behavior of the smallest particles and the larger particles during actual translocation suggest that the fundamental processes in the mechanisms of organelle movement in axonal transport are not saltatory but continuous.