Histatin and lactoferrin derived peptides: Antimicrobial properties and effects on mammalian cells
Histatin and lactoferrin derived peptides: Antimicrobial properties and effects on mammalian cells
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DOI：
10.1016/j.peptides.2005.05.014
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发表时间：
2005-12-01
期刊：
影响因子：
3
通讯作者：
Wuisman, PIJM
中科院分区：
文献类型：
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作者：
Stallmann, HP;Faber, C;Wuisman, PIJM
为了分析两种抗菌肽，人乳铁蛋白1-11（hLF 1 - 11）和合成的组胺素类似物Dhvar-5的临床潜力，我们测量了对细菌的杀伤作用以及对红细胞和骨细胞的潜在毒性。在对包括耐甲氧西林金黄色葡萄球菌在内的六种菌株的杀灭试验中测定了抗微生物活性。分别采用溶血试验和活力试验测量对人红细胞和MC 3 T3小鼠骨细胞的影响。hLF 1 -11和Dhvar-5都以6 μ g/mL的浓度开始剂量依赖性地杀死所有细菌菌株。hLF 1 -11在浓度高达400 μ g/mL时对哺乳动物细胞没有影响，但Dhvar-5诱导了显著的溶血（200 μ g/mL时为37%）和骨细胞死亡（400 μ g/mL时为70%）。这表明这两种肽都能够杀死各种耐药和非耐药细菌，但Dhvar-5可能在较高浓度下对宿主细胞产生细胞毒性作用。(c)2005年爱思唯尔公司All rights reserved.
In order to analyze the clinical potential of two antimicrobial peptides, human lactoferrin 1-11 (hLF1 -11) and synthetic histatin analogue Dhvar-5, we measured the killing effect on bacteria, and the potential toxicity on erythrocytes and bone cells. The antimicrobial activity was determined in a killing assay on six strains, including methicillin resistant Staphylococcus Aureus. The effect on human erythrocytes and MC3T3 mouse bone cells was measured with a hemolysis assay and a viability assay, respectively. Both hLF1-11 and Dhvar-5 dose-dependently killed all bacterial strains, starting at concentrations of 6 mu g/mL. hLF1-11 had no effect on mammalian cells at concentrations up to 400 mu g/mL, but Dhvar-5 induced significant hemolysis (37% at 200 mu g/mL) and bone cell death (70% at 400 mu g/mL). This indicates that both peptides are able to kill various resistant and non-resistant bacteria, but Dhvar-5 may exert a cytotoxic effect on host cells at higher concentrations. (c) 2005 Elsevier Inc. All rights reserved.