Mouse Models of Accelerated Cellular Senescence.
Mouse Models of Accelerated Cellular Senescence.
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DOI：
10.1007/978-1-4939-8931-7_17
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Niedernhofer LJ
中科院分区：
文献类型：
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作者：
Yousefzadeh MJ;Melos KI;Angelini L;Burd CE;Robbins PD;Niedernhofer LJ
随着几乎所有脊椎动物的衰老，衰老细胞在多种组织中积累。衰老是对许多形式的细胞应激的高度保守的反应，旨在阻止受损细胞的繁殖。已证明衰老细胞通过其衰老相关的分泌表型和通过阻碍组织再生在衰老中发挥因果作用。通过遗传或药理学方法消耗衰老细胞已被证明可以延长小鼠寿命并延迟年龄相关疾病的发作。测量体内衰老细胞的负荷和位置仍然具有挑战性，因为没有衰老细胞特有的标记物。在这里，我们描述了多种方法来检测临床前模型中细胞衰老的存在和程度，特别强调加速衰老的小鼠模型，其表现出更快的细胞衰老开始。
Senescent cells accumulate in multiple tissues as virtually all vertebrate organisms age. Senescence is a highly conserved response to many forms of cellular stress intended to block the propagation of damaged cells. Senescent cells have been demonstrated to play a causal role in aging via their senescence-associated secretory phenotype and by impeding tissue regeneration. Depletion of senescent cells either through genetic or pharmacologic methods has been demonstrated to extend murine lifespan and delay the onset of age-related diseases. Measuring the burden and location of senescent cells in vivo remains challenging, as there is no marker unique to senescent cells. Here, we describe multiple methods to detect the presence and extent of cellular senescence in pre-clinical models, with a special emphasis on murine models of accelerated aging that exhibit a more rapid onset of cellular senescence.