Characterization and antioxidant activity of bovine serum albumin and sulforaphane complex in different solvent systems
Characterization and antioxidant activity of bovine serum albumin and sulforaphane complex in different solvent systems
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DOI：
10.1016/j.jlumin.2013.09.078
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发表时间：
2014-02
影响因子：
3.6
通讯作者：
Xueyan Dong;Rui Zhou;H. Jing
中科院分区：
文献类型：
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作者：
Xueyan Dong;Rui Zhou;H. Jing
研究牛血清白蛋白（BSA）与萝卜硫素（SFN）的相互作用方式及其影响因素，有助于了解生物活性小分子和生物大分子的相互作用机制和功能变化。采用傅里叶变换红外光谱（FT-IR）、荧光光谱、同步荧光光谱、DPPH和ABTS自由基清除实验等方法，研究了BSA和SFN在去离子水（dH 2 O）、二甲基亚砜（DMSO）和乙醇（EtOH）3种溶剂体系中的相互作用机制及其抗氧化活性。结果表明，SFN在静态条件下能猝灭BSA的荧光，并能在酪氨酸（Tyr）和色氨酸（Trp）残基上与BSA相互作用，其中色氨酸残基具有较高的灵敏度，在340 nm处的荧光强度证明了这一点。疏水力、氢键和货车范德华力均参与了BSA与SFN的相互作用，但三种溶剂对BSA与SFN的相互作用影响不显著。结合常数和结合位点数的大小顺序为dH_2O>DMSO>EtOH。自由能变化值的大小顺序为dH_2O>DMSO>EtOH，说明结合力的大小顺序为dH_2O>DMSO>EtOH。SFN和BSA SFN的抗氧化活性无显著差异。三种溶剂对SFN和BSA-SFN的抗氧化活性影响不显著。
Modes and influencing factors of bovine serum albumin (BSA) and sulforaphane (SFN) interaction will help us understand the interaction mechanisms and functional changes of bioactive small molecule and biomacromolecule. This study investigated interaction mechanisms of BSA and SFN and associated antioxidant activity in three solvent systems of deionized water (dH2O), dimethyl sulfoxide (DMSO) and ethanol (EtOH), using Fourier transform infrared spectroscopy (FT-IR), fluorescence spectroscopy, synchronous fluorescence spectroscopy, DPPH and ABTS radical scavenging assays. The results revealed that SFN had ability to quench BSA's fluorescence in static modes, and to interact with BSA at both tyrosine (Tyr) and tryptophan (Trp) residues, while the Trp residues were highly sensitive, which was demonstrated by fluorescence at 340 nm. Hydrophobic forces, hydrogen bonds and van der Waals interactions were all involved in BSA and SFN interaction, which were not significantly changed by three solvents. The binding constant values and binding site numbers were in a descending order of dH2O>DMSO>EtOH. The values of free energy change were in a descending order of dH2O>DMSO>EtOH, which indicated that the binding forces were in a descending order of dH2O>DMSO>EtOH. There was no significant difference in antioxidant activity between SFN and BSA–SFN. Moreover, three solvents had not significant influence on antioxidant activity of SFN and BSA–SFN.