Lens-derived Sernaphorin3A regulates sensory innervation of the cornea
Lens-derived Sernaphorin3A regulates sensory innervation of the cornea
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DOI：
10.1016/j.ydbio.2007.04.012
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发表时间：
2007-06-15
影响因子：
2.7
通讯作者：
Bronner-Fraser, Marianne
中科院分区：
文献类型：
--
作者：
Lwigale, Peter Y.;Bronner-Fraser, Marianne
角膜是人体神经支配最多的组织之一，受三叉神经感觉传入神经支配。在发育过程中，轴突最初在角膜边缘被排斥，导致。周围神经环的堡垒调节这一过程的引导分子的性质和来源仍然是一个谜。在这里，我们表明，透镜，直接下的角膜，排斥三叉神经轴突在体内和体外。透镜消融导致过早的、紊乱的角膜神经支配和神经环和腹丛的破坏。我们发现，Semaphorin 3A（Sema 3A）的表达在透镜上皮细胞和其受体Neuropilin-1（Npn 1）的三叉神经节在角膜发育过程中表达。Sema 3A信号传导的抑制在体外消除了透镜和角膜的轴突排斥，在体内消除了透镜的表型。这些结果表明，晶状体衍生的Sema 3A介导三叉神经感觉轴突从角膜的初始排斥，并且是必要的神经环的正确形成和定位的腹丛在脉络膜裂。(C)2007年爱思唯尔公司All rights reserved.
The cornea, one of the most highly innervated tissues of the body, is innervated by trigeminal sensory afferents. During development, axons are initially repelled at the corneal margin, resulting in. the fort-nation of a circumferential nerve ring. The nature and source of guidance molecules that regulate this process remain a mystery. Here, we show that the lens, which immediately underlies the cornea, repels trigeminal axons in vivo and in vitro. Lens ablation results in premature, disorganized corneal innervation and disruption of the nerve ring and ventral plexus. We show that Semaphorin3A (Sema3A) is expressed in the lens epithelium and its receptor Neuropilin-1 (Npn1) is expressed in the trigeminal ganglion during cornea development. Inhibition of Sema3A signaling abrogates axon repulsion by the lens and cornea in vitro and phenocopies lens removal in vivo. These results demonstrate that lens-derived Sema3A mediates initial repulsion of trigeminal sensory axons from the cornea and is necessary for the proper formation of the nerve ring and positioning of the ventral plexus in the choroid fissure. (C) 2007 Elsevier Inc. All rights reserved.