The transformation booster sequence from Petunia hybrida is a retrotransposon derivative that binds to the nuclear scaffold
The transformation booster sequence from Petunia hybrida is a retrotransposon derivative that binds to the nuclear scaffold
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来自矮牵牛的转化加强序列是一种逆转录转座子衍生物，可与核支架结合
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
P. Meyer
中科院分区：
文献类型：
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作者：
H. Galliano;A. Müller;J. M. Lucht;P. Meyer
来自矮牵牛的转化增强序列（TBS）提高了在矮牵牛、烟草和玉米中的转化频率。TBS还刺激P.hybrida中的同源分子间和分子内重组，这种刺激的分子基础尚不清楚。我们研究了TBS是否含有可能有助于刺激重组的序列元件，以及其重组潜力是否反映了TBS的生物学功能。我们确定了TBS内的支架附着区（SAR），并分析了其在基因组中的分布及其与其他基因组序列的同源性。TBS的A516亚片段与核支架结合。TBSSAR片段的序列显示与来自植物的逆转录病毒元件具有很强的同源性，表明TBS是逆转录病毒的无活性衍生物，其仍然促进DNA重组。
The transformation booster sequence (TBS) from Petunia hybrida enhances transformation frequencies in P. hybrida, Nicotiana tabacum and Zea mays. TBS also stimulates homologous inter- and intramolecular recombination in P. hybrida, the molecular basis for this stimulation is not known. We investigated whether TBS contains sequence elements that might contribute to the stimulation of recombination and whether its recombinogenic potential reflects a biological function of TBS. We identified a scaffold attachment region (SAR) within TBS and analysed its distribution in the genome and its homologies to other genomic sequences. A 516 by subfragment of TBS binds to the nuclear scaffold. The sequence of the TBSSAR fragment shows strong homologies to retroviral elements from plants, suggesting that TBS is an inactive derivative of a retrovirus that still promotes DNA recombination.
DNA 拓扑异构酶和姐妹染色单体交换模型。
DOI：
10.1016/0027-5107(89)90213-3
发表时间：
1989
期刊：
Mutation research
影响因子：
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作者：
Dillehay,LE;Jacobson-Kram,D;Williams,JR
通讯作者：
Williams,JR