Allele-Specific Inhibition of Histone Demethylases.
Allele-Specific Inhibition of Histone Demethylases.
复制标题
组蛋白去甲基酶的等位基因特异性抑制。
DOI：
10.1002/cbic.201800756
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Islam,Kabirul
中科院分区：
文献类型：
--
作者：
Wagner,Shana;Waldman,Megan;Arora,Simran;Wang,Sinan;Scott,Valerie;Islam,Kabirul
Histone demethylases play a critical role in mammalian gene expression by removing methyl groups from lysine residues in degree‐ and site‐specific manner. To specifically interrogate members and isoforms of this class of enzymes, we have developed demethylase variants with an expanded active site. The mutant enzymes are capable of performing lysine demethylation with wild‐type proficiency, but are sensitive to inhibition by cofactor‐competitive molecules embellished with a complementary steric “bump”. The selected inhibitors show more than 20‐fold selectivity over the wild‐type demethylase, thus overcoming issues typical to pharmacological and genetic approaches. The mutant–inhibitor pairs are shown to act on a physiologically relevant full‐length substrate. By engineering a conserved amino acid to achieve member‐specific perturbation, this study provides a general approach for studying histone demethylases in diverse cellular processes.
