DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes
DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes
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DOI：
10.1128/iai.70.6.3300-3303.2002
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发表时间：
2002-06-01
影响因子：
3.1
通讯作者：
Fikrig, E
中科院分区：
文献类型：
--
作者：
Liang, FT;Nelson, FK;Fikrig, E
用含有137个伯氏疏螺旋体B31推定脂蛋白基因片段的DNA微阵列检测莱姆病螺旋体。来自B的DNA狭义伯氏疏螺旋体B31、297和N40;伽氏疏螺旋体IP 90;和阿氏疏螺旋体P/Gau荧光标记并与微阵列杂交，证明了各个推定的脂蛋白基因在基因种中保守的程度。这些数据表明，DNA微阵列可以全面检查编码B的基因。伯氏脂蛋白
A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.