Bone morphogenetic protein-2, but not bone morphogenetic protein-7, promotes dendritic growth and calbindin phenotype in cultured rat striatal neurons
Bone morphogenetic protein-2, but not bone morphogenetic protein-7, promotes dendritic growth and calbindin phenotype in cultured rat striatal neurons
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DOI：
10.1016/s0306-4522(01)00122-1
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Alberch, J
中科院分区：
文献类型：
--
作者：
Gratacòs, E;Checa, N;Alberch, J
骨形态发生蛋白是转化生长因子-β超家族的成员。它们在哺乳动物神经系统中广泛表达，对多个神经元群体产生营养作用。我们研究了骨形态发生蛋白 2 和骨形态发生蛋白 7（也称为成骨蛋白 1）对培养的纹状体细胞的神经营养活性，先前显示这些细胞表达骨形态发生蛋白配体和受体。我们的结果表明，只有骨形态发生蛋白-2 促进 GABA 能神经元的分化，尤其是钙结合蛋白阳性亚群的分化，这是在亨廷顿病中退化的投射纹状体神经元的亚群。骨形态发生蛋白2增加了面积。 GABA能神经元的树状化周长和程度，促进钙结合蛋白表型而不改变增殖或凋亡。相比之下，野猪形态发生蛋白-2和-7都不影响纹状体胆碱能中间神经元。然而，它们都增加了胶质纤维的数量？酸性蛋白阳性细胞。用5-氟脱氧尿苷抑制胶质细胞增殖并不能消除骨形态发生蛋白2对纹状体神经元分化的影响，排除了通过星形胶质细胞的间接机制。 总之，我们的结果表明骨形态发生蛋白2促进培养的GABA能纹状体神经元的分化，表明骨形态发生蛋白参与纹状体的发育。 (C) 2001 国际广播组织。由爱思唯尔科学有限公司出版。保留所有权利。
Bone morphogenetic proteins are members of the transforming growth factor-beta superfamily. They are widely expressed in the mammalian nervous system, where thr) evert trophic effects on several neuronal populations. We studied the neurotrophic activity of bone morphogenetic protein-2 and bone morphogenetic protein-7 (also called osteogenic protein-1) on cultured striatal cells, previously shown to express bone morphogenetic protein ligands and receptors. Our results indicate that only bone morphogenetic protein-2 promoted the differentiation of GABAergic neurons, especially of the calbindin-positive subpopulation, the subset of projecting striatal neurons that degenerates in Huntington's disease. Bone morphogenetic protein-2 increased the area. perimeter and degree of arborization of GABAergic neurons, promoting calbindin phenotype without altering proliferation or apoptosis. In contrast, neither boar morphogenetic protein-2 nor -7 affected striatal cholinergic interneurons. However, they both increased the number of glial fibrillar?; acidic protein-positive cells. Suppression of glial proliferation with 5-fluorodeoxyuridine did not abolish bone morphogenetic protein-2 effects on the differentiation of striatal neurons, ruling out an indirect mechanism through astrocytes.In conclusion, our results show that bone morphogenetic protein-2 promotes the differentiation of cultured GABAergic striatal neurons, suggesting that bone morphogenetic proteins are involved in the development of the striatum. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.