New Aequorea Fluorescent Proteins for Cell-Free Bioengineering
New Aequorea Fluorescent Proteins for Cell-Free Bioengineering
复制标题
DOI：
10.1021/acssynbio.3c00057
复制
发表时间：
2023-04-05
影响因子：
4.7
通讯作者：
Adamala, Katarzyna P.
中科院分区：
文献类型：
--
作者：
Deich, Christopher;Gaut, Nathaniel J.;Adamala, Katarzyna P.
最近，一个新的荧光蛋白亚类已经从水母中鉴定出来。这些荧光蛋白的特点是在体内，但是，还没有验证这些蛋白质在无细胞系统。无细胞系统和技术开发是一个快速发展的领域，包括基础研究，合成细胞，生物工程，生物制造和药物开发。无细胞系统严重依赖荧光蛋白作为报告基因。在这里，我们表征和验证了这组新的水母蛋白质用于各种无细胞和合成细胞表达平台。
Recently, a new subset of fluorescent proteins has been identified from the Aequorea species of jellyfish. These fluorescent proteins were characterized in vivo; however, there has not been validation of these proteins within cell-free systems. Cell-free systems and technology development is a rapidly expanding field, encompassing foundational research, synthetic cells, bioengineering, biomanufacturing, and drug development. Cell-free systems rely heavily on fluorescent proteins as reporters. Here we characterize and validate this new set of Aequorea proteins for use in a variety of cell-free and synthetic cell expression platforms.