8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in human urine do not depend on diet
8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in human urine do not depend on diet
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DOI：
10.1080/10715760100301321
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发表时间：
2001-01-01
影响因子：
3.3
通讯作者：
Olinski, R
中科院分区：
文献类型：
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作者：
Gackowski, D;Rozalski, R;Olinski, R
本研究采用HPLC预纯化-气相色谱-同位素稀释质谱法检测人尿中8-氧-7,8-二氢-2'-脱氧鸟苷（8-oxodGua）和8-氧-7,8-二氢鸟嘌呤（8-oxoGua）。无限制饮食组受试者尿液样本中8-氧瓜和8-氧过的平均水平分别为1.87 nmol/kg 24h（+/-0.90）和0.83 nmol/kg 24h(+/-0.49)，并且在研究组的情况下，它们不依赖于应用饮食。尿液中这两种化合物的总量可以提供细胞DNA中8-氧瓜形成速率的信息。尿液样本中修饰的核碱基/侧的水平也可能反映了负责从DNA中去除8-oxodGuo的不同修复途径的参与，即碱基切除修复（BER）和核苷酸切除修复（NER）。
In the present study, we used the method involving HPLC pre-purification followed by gas chromatography with isotope dilution mass spectrometric detection for the determination of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGua) and 8-oxo-7,8-dihydroguanine (8-oxoGua) in human urine. The mean levels of 8-oxoGua and 8-oxodGuo in the urine samples of the subjects on unrestricted diet were respectively 1.87 nmol/kg 24h (+/-0.90) and 0.83 nmol/kg 24h (+/-0.49), and in the case of the groups studied, they did not depend on the applied diet. The sum of the amounts of both compounds in urine can give information about the formation rate of 8-oxoGua in cellular DNA. It is also likely that the levels of modified nucleo-base/side in urine sample are reflective of the involvement of different repair pathways responsible for the removal of 8-oxodGuo from DNA, namely base excision repair (BER) and nucleotide excision repair (NER).