Targeted disruption in the mouse Hoxc-4 locus results in axial skeleton homeosis and malformation of the xiphoid process.
Targeted disruption in the mouse Hoxc-4 locus results in axial skeleton homeosis and malformation of the xiphoid process.
复制标题
小鼠 Hoxc-4 基因座的靶向破坏导致轴骨同质异形和剑突畸形。
DOI：
10.1006/dbio.1996.0051
复制
发表时间：
1996
影响因子：
2.7
通讯作者：
Hirofumi Suemori
中科院分区：
文献类型：
--
作者：
Hironao Saegusa;Naoki Takahashi;Shigeru Noguchi;Hirofumi Suemori
HoxC-4是一个小鼠同源盒基因，位于HoxC簇的3‘端。在HoxC基因中，Hoxc-4在中枢神经系统的最前部和脊柱前表达。为了研究它在小鼠发育中的作用，我们通过基因打靶产生了Hoxc-4突变小鼠。携带HOXC-4基因突变纯合子的小鼠是有活力和生育能力的。对纯合子突变的骨骼系统的分析显示，颈部和胸部有各种异常。最常见的异常是第七颈椎的部分后方顺应性改变。较少见的是，观察到第三和第八胸椎的前移。此外，胸骨的剑突畸形，以致它有一个孔或裂隙。虽然HOXC-4在中枢神经系统中大量表达，但没有观察到明显的缺陷。这些结果表明，Hoxc-4是确定颈椎和胸椎特性所必需的。
Hoxc-4 is a mouse homeobox gene located at the 3' end of the HoxC cluster. Of the HoxC genes, Hoxc-4 is expressed in the most anterior regions of the central nervous system and prevertebral column. To investigate its role in mouse development, we have generated Hoxc-4 mutant mice by gene targeting. Mice homozygous for the Hoxc-4 mutation are viable and fertile. Analysis of the skeletal system of homozygous mutants revealed various abnormalities in the cervical and thoracic regions. The most frequent abnormality was a partial posterior homeotic transformation of the seventh cervical vertebra. Less frequently, anterior transformations of the third and eighth thoracic vertebrae were observed. Furthermore, the xiphoid process of the sternum was malformed such that it had an aperture or a fissure. Although Hoxc-4 is expressed abundantly in the central nervous system, no obvious defects were observed. These results suggest that Hoxc-4 is required for specifying cervical and thoracic vertebral identity.