A cloned human T cell line cytotoxic for autologous and allogeneic B lymphoma cells.
A cloned human T cell line cytotoxic for autologous and allogeneic B lymphoma cells.
复制标题
克隆的人T细胞系细胞毒性，用于自体和同种异体B淋巴瘤细胞。
DOI：
10.1084/jem.160.1.239
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发表时间：
1984-07-01
影响因子：
15.3
通讯作者：
de Vries, J E
中科院分区：
文献类型：
--
作者：
Yssel, H;Spits, H;de Vries, J E
通过用新鲜自体淋巴瘤细胞重复刺激，在混合肿瘤细胞培养物中的无血清培养基中建立具有自体肿瘤反应性的人细胞毒性T细胞克隆（MWS-14）。该克隆及其亚克隆具有T3+ T4+ T8-表型。它们对自体淋巴瘤细胞有很强的细胞毒性，而自体PHA母细胞没有被杀死。MWS-14、MWS-14- 30和MWS-14-34的特异性分析表明，这些CTL克隆对7/7的同种异体淋巴瘤细胞具有细胞毒性，而仅3/23的正常和非淋巴瘤细胞被裂解。针对MHC I类和II类抗原的单克隆抗体阻断研究表明，这种优先的抗淋巴瘤反应性不针对HLA决定簇。抗淋巴瘤活性不是由于淋巴瘤细胞对溶解的非特异性易感性。与对淋巴瘤细胞上存在的HLA抗原具有特异性的CTL克隆相反，T3和T4不参与MWS-14对自体淋巴瘤细胞的细胞毒性反应。该克隆的反应性可被针对白细胞功能相关抗原的单克隆抗体阻断。从这些结果可以得出结论，这些T4+ CTL克隆识别决定簇，其优先在自体和同种异体淋巴瘤细胞上表达。
A human cytotoxic T cell clone (MWS-14) with auto-tumor reactivity was established in serum-free medium in a mixed tumor cell culture by repetitive stimulation with fresh autologous lymphoma cells. This clone and its subclones are of the T3+ T4+ T8- phenotype. They were strongly cytotoxic for the autologous lymphoma cells, whereas autologous PHA blasts were not killed. Analysis of the specificity of MWS-14, MWS-14- 30, and MWS-14-34 indicated that these CTL clones were cytotoxic for 7/7 allogeneic lymphoma cells, whereas only 3/23 of normal and non- lymphoma cells were lysed. Blocking studies with monoclonal antibodies directed at MHC class I and class II antigens showed that this preferential, anti-lymphoma reactivity was not directed at HLA determinants. The anti-lymphoma activity is not due to an aspecific susceptibility of the lymphoma cells to lysis. In contrast to CTL clones specific for HLA antigens present on the lymphoma cells, T3 and T4 were not involved in the cytotoxic reaction of MWS-14 against the autologous lymphoma cells. The reactivity of this clone could be blocked by a monoclonal antibody directed at leukocyte function- associated antigen. It can be concluded from these results that these T4+ CTL clones recognize a determinant, which is preferentially expressed on autologous and allogeneic lymphoma cells.