Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization
Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization
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DOI：
10.1016/j.bone.2006.02.059
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发表时间：
2006-09-01
期刊：
影响因子：
4.1
通讯作者：
Ignelzi, Michael A., Jr.
中科院分区：
文献类型：
--
作者：
Crane, Nicole J.;Popescu, Victoria;Ignelzi, Michael A., Jr.
为了了解早期矿化事件，我们使用成纤维细胞生长因子 2 (FGF2) 浸泡的多孔珠通过拉曼光谱研究了活体小鼠颅骨组织。我们在过早缝合缝合线中检测到类似于磷酸八钙的瞬态相水平增加。简介：几种磷酸钙已被认为是骨矿物质的最早的无机前体。它们不稳定，之前从未在组织样本中检测到。是否在过早闭合的缝线中形成相同的中间体也是未知的。方法：对来自胎儿18.5天的B6CBA F-1/J野生型小鼠的6个冠状缝组织标本进行研究。使用 FGF2 浸泡的肝素丙烯酸珠处理三个缝合线标本，以诱导加速矿化和过早缝合线闭合。用空的肝素丙烯酸珠处理三个对照样本。所有缝合线均保持为器官培养物，以便在 72 小时内以 12-24 小时的间隔重复进行光谱分析。结果：在前 24 小时内，光谱包含磷酸八钙 (OCP) 或类似 OCP 的矿物质的条带。主要的磷氧拉伸位于 955 cm(-1)，而不是骨矿物质中看到的 957-959 cm(-1)，并且在 1010-1014 cm(-1) 处有一条附加带，正如 OCR 所预期的那样。在 945 cm(-1) 处发现了一条宽带，这是高度无序或无定形磷酸钙的特征。在经 FGF2 处理的缝合线中观察到矿物质含量增加，但在实验样本和对照样本之间未观察到拉曼光谱的质量差异。结论：无机矿物质沉积通过瞬态中间体（包括 OCP 样相）进行。尽管这种瞬态相已在纯无机模型系统中观察到，但这项研究首次报告了活体组织中的 OCP 或类似 OCP 的中间体。拉曼显微光谱可以观察这种瞬态矿物，也可以观察其他前体。 (c) 2006 Elsevier Inc. 保留所有权利。
To understand early mineralization events, we studied living murine calvarial tissue by Raman spectroscopy using fibroblast growth factor 2 (FGF2)-soaked porous beads. We detected increased levels of a transient phase resembling octacalcium phosphate in sutures undergoing premature suture closure.Introduction: Several calcium phosphates have been postulated as the earliest inorganic precursors to bone mineral. They are unstable and have not been previously detected in tissue specimens. Whether the same intermediates are formed in sutures undergoing premature closure is also unknown.Methods: Six coronal suture tissue specimens from fetal day 18.5 B6CBA F-1/J wild-type mice were studied. Three sutures specimens were treated with FGF2-soaked heparin acrylic beads to induce accelerated mineralization and premature suture closure. Three control specimens were treated with empty heparin acrylic beads. All sutures were maintained as organ cultures to permit repeated spectral analyses at 12-24 h intervals over a 72-h period.Results: During the first 24 h, the spectra contained bands of octacalcium phosphate (OCP) or an OCP-like mineral. The main phosphorus-oxygen stretch was at 955 cm(-1), instead of the 957-959 cm(-1) seen in bone mineral, and there was an additional band at 1010-1014 cm(-1), as expected for OCR A broad band was found at 945 cm(-1) characteristic of a highly disordered or amorphous calcium phosphate. An increased amount of mineral was observed in FGF2-treated sutures, but no qualitative differences in Raman spectra were observed between experimental and control specimens.Conclusions: Inorganic mineral deposition proceeds through transient intermediates, including an OCP-like phase. Although this transient phase has been observed in purely inorganic model systems, this study is the first to report OCP or an OCP-like intermediate in living tissue. Raman microspectroscopy allows observation of this transient mineral and may allow observation of other precursors as well. (c) 2006 Elsevier Inc. All rights reserved.