High incidence of Zika virus infection detected in plasma and cervical cytology specimens from pregnant women in Guayaquil, Ecuador.
High incidence of Zika virus infection detected in plasma and cervical cytology specimens from pregnant women in Guayaquil, Ecuador.
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在厄瓜多尔瓜亚基尔孕妇的血浆和宫颈细胞学标本中检测到寨卡病毒感染率很高。
DOI：
10.1111/aji.12630
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Mor,Gil
中科院分区：
文献类型：
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作者：
Zambrano,Hector;Waggoner,Jesse;León,Karina;Pinsky,Benjamin;Vera,Ketty;Schettino,Marissa;Rivera,Lisette;Landivar,José;Granda,María;Lee,Angela;Mor,Gil
孕期感染寨卡病毒(ZIKV)与严重的出生缺陷有关，厄瓜多尔ZIKV疫情的流行病学情况知之甚少。厄瓜多尔瓜亚基尔属热带气候，登革热和基孔肯雅病毒频繁爆发，2015年12月发现寨卡病毒。考虑到ZIKV对妊娠的影响，包括小头畸形，我们检测了孕妇血浆和宫颈细胞学中ZIKV的存在。我们报告了一组在血浆和下生殖道中检测到ZIKV感染高发的孕妇。进行了一项病例对照研究，以确定低收入、有早产风险的孕妇与匹配的对照组相比ZIKV感染的发生率。血浆和宫颈细胞学标本用RRT-PCR检测ZIKV。59名孕妇参加了调查。ZIKV的总发生率为54%(32/59)：病例组18/31(58.1%)，对照组14/28(50.0%)。在血浆和宫颈细胞学标本中检测ZIKV具有很好的一致性。总体而言，ZIKV阳性母亲和ZIKV阴性母亲所生新生儿的结局是相似的。然而，两名出生时患有小头畸形症的母亲是ZIKV阳性的母亲。我们报告了厄瓜多尔瓜亚基尔一个特殊人群中ZIKV感染的高发病率(54%)。我们在宫颈样本中发现ZIKV与血浆中的ZIKV相关。这些数据引起了人们对厄瓜多尔寨卡病毒流行范围的关注，并证明了宫颈细胞学标本对寨卡病毒检测的实用性。
Zika virus (ZIKV) infection during pregnancy has been linked to severe birth defects, and the epidemiologic situation of the ZIKV epidemic in Ecuador is poorly understood. Guayaquil, Ecuador, has a tropical climate and experiences frequent outbreaks of dengue and chikungunya virus, and in December 2015, ZIKV was identified. Given the well‐documented effects of ZIKV in pregnancy, including microcephaly, we tested for the presence of ZIKV in both plasma and cervical cytology of pregnant women. We report the identification of a population of pregnant women with a high incidence of ZIKV infection detected in the plasma and lower reproductive tract. A case‐control study was performed to determine the incidence of ZIKV infection among low‐income, pregnant women at risk for preterm delivery compared to matched controls. Plasma and cervical cytology specimens were tested for ZIKV by rRT‐PCR. Fifty‐nine pregnant women were enrolled. The incidence of ZIKV was 54% (32/59) overall: 18/31 (58.1%) in cases and 14/28 (50.0%) in controls. ZIKV detection in plasma and cervical cytology specimens demonstrated good agreement. Overall, outcomes for neonates born to ZIKV‐positive and ZIKV‐negative mothers were similar. However, two neonates were born with microcephaly to case mothers who were ZIKV positive. We report a high incidence of ZIKV infection (54%) in a distinctive population in Guayaquil, Ecuador. We identify ZIKV in cervical samples that correlates with ZIKV in the plasma. These data raise concerns regarding the breadth of the ZIKV epidemic in Ecuador and demonstrate the utility of cervical cytology specimens for ZIKV testing.