Chicken NK-lysin is an alpha- helical cationic peptide that exerts its antibacterial activity through damage of bacterial cell membranes
Chicken NK-lysin is an alpha- helical cationic peptide that exerts its antibacterial activity through damage of bacterial cell membranes
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DOI：
10.3382/ps.2013-03670
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发表时间：
2014-04-01
期刊：
影响因子：
4.4
通讯作者：
Womack, James E.
中科院分区：
文献类型：
--
作者：
Lee, Mi Ok;Jang, Hyun-Jun;Womack, James E.
The antimicrobial peptides (AMP) are important elements of the first line of defense against pathogens in animals, and an important constituent of innate immunity. Antimicrobial peptides act on a broad spectrum of microbial organisms. NK-Lysin is a cationic antibacterial peptide that was originally isolated from porcine intestinal tissue based on its antibacterial activity. We synthesized peptides corresponding to each helical region of chicken NK-lysin and analyzed their secondary structures in addition to their antimicrobial activity. Circular dichroism spectroscopy of the synthetic chicken NK-lysin (cNK-78) and 4 small peptides in negatively charged liposomes demonstrated transition in the conformation of a-helical peptides relative to the charged environment. Chicken NK-lysin inhibits the growth of a representative gram-negative bacterium, Escherichia coli. The antimicrobial activity of 2 peptides designated H23 and H34 was similar to that of mature NK-lysin, cNK-78. Microscopic analyses revealed the death of bacterium with disrupted membranes after peptide treatment, suggesting that chicken NK-lysin, an alpha-helical cationic peptide, exerts its antimicrobial activity by damaging the bacterial cell membrane.
