Enhanced biodegradation of pentachlorophenol in unsaturated soil using reversed field electrokinetics.
Enhanced biodegradation of pentachlorophenol in unsaturated soil using reversed field electrokinetics.
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DOI：
10.1016/j.jenvman.2008.12.012
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发表时间：
2009-04
影响因子：
8.7
通讯作者：
M. Harbottle;G. Lear;G. Sills;I. Thompson
中科院分区：
文献类型：
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作者：
M. Harbottle;G. Lear;G. Sills;I. Thompson
研究了在非饱和土壤中利用电动力学通过增加细菌与污染物之间的接触来促进五氯苯酚的生物降解。对被约100 mg kg− 1五氯苯酚（含有[14 C]-五氯苯酚作为示踪剂）污染并接种特定五氯苯酚降解菌（鞘氨醇菌属UG 30 -1 × 108 cfu g−1）的土壤微生态系统施加恒定和定期反向电流（10 mA）。前者由于水电解和电渗效应导致pH值和水分含量发生较大变化，随后对生物降解参数（包括酶活性和污染物矿化（通过14 CO2释放速率测量））产生负面影响。反向场引起的pH值和水分含量的变化不大，并导致更快的污染物矿化，降低土壤污染物浓度在大多数的微观世界和增加土壤酶活性（除了紧邻阳极的土壤）。因此，电场的存在，如果适当地施加，可以提高污染物在非饱和土壤中的生物降解。
This study investigated the use of electrokinetics in unsaturated soil to promote biodegradation of pentachlorophenol through increased contact between bacteria and contaminant. Soil microcosms, contaminated with approximately 100 mg kg−1pentachlorophenol (containing [14C]-PCP as a tracer), and inoculated with a specific pentachlorophenol-degrading bacterium (Sphingobium sp. UG30–1 × 108cfu g−1) were subjected to constant and regularly reversed electric currents (10 mA). The former caused large pH and moisture content changes due to water electrolysis and electroosmotic effects, with subsequent negative impacts on biodegradation parameters including enzyme activity and contaminant mineralisation (as measured by14CO2evolution rate). The reversed field caused little change in pH and moisture content and led to more rapid contaminant mineralisation, lower soil contaminant concentration in the majority of the microcosms and increased soil enzyme activity (with the exception of soil immediately adjacent to the anode). The presence of an electric field, if suitably applied, may therefore enhance contaminant biodegradation in unsaturated soil.