PATHWAYS OF CELLULAR EFFLUX AND PARTICULATE CLEARANCE AFTER CARBON INSTILLATION TO THE LUNG
PATHWAYS OF CELLULAR EFFLUX AND PARTICULATE CLEARANCE AFTER CARBON INSTILLATION TO THE LUNG
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DOI：
10.1002/path.1711430206
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发表时间：
1984-01-01
影响因子：
7.3
通讯作者：
ADAMSON, IYR
中科院分区：
文献类型：
--
作者：
BOWDEN, DH;ADAMSON, IYR
研究了肺对碳颗粒沉积的反应，以确定细胞外排的途径和颗粒清除的机制。气管内灌注4mg碳后，每隔一段时间至6mo，灌注固定小鼠肺，不灌洗。在数小时内，观察到粒细胞向细支气管内迁移；肺泡内直到24小时才出现大量PMN[多形核细胞]。与PMN外排相关的是短暂性水肿，没有肺细胞坏死的证据。游离巨噬细胞的数量随着碳细胞和单核细胞向肺泡和细支气管的迁移而增加。细胞外排的细支气管成分表明，通过灌洗从肺中恢复的PMN和巨噬细胞并不完全来自肺泡。虽然大多数颗粒，无论是游离的还是吞噬细胞中的，都被支气管树清除，但观察到一些游离颗粒通过上皮进入间质。在肺门淋巴结中发现一些碳，但整体淋巴清除率低。[描述组织学变化]。
The pulmonary response to the deposition of carbon particles was investigated to determine the routes of cellular efflux and the mechanisms of particulate clearance. At intervals to 6 mo. after the intratracheal instillation of 4 mg carbon, the lungs of mice were fixed in situ by perfusion without lavage. Within a few hours, migration of granulocytes into the bronchioles was observed; large numbers of PMN [polymorphonuclear cells] were not seen in alveoli until 24 h. Associated with the PMN efflux there was transient edema with no evidence of pulmonary cell necrosis. The number of free macrophages increased in response to the carbon and mononuclear cell migration into alveoli and bronchioles was also observed. The bronchiolar component of the cellular efflux indicates that PMN and macrophages recovered from the lung by lavage are not entirely of alveolar origin. Whereas most particles, either free or in phagocytic cells, were cleared by the bronchial tree, some transepithelial passage of free particles to the interstitium was observed. Some carbon was found in hilar lymph nodes but overall lymphatic clearance was low. [Histologic changes are described.].