Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis
Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis
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DOI：
10.1016/j.jri.2005.11.003
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发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Dara誰, Emile
中科院分区：
文献类型：
--
作者：
Dufournet, Charlotte;Uzan, Catherine;Dara誰, Emile
子宫内膜异位症被定义为子宫内膜腺体和间质存在于子宫外。细胞凋亡是多细胞生物消除多余细胞的生理过程，在肿瘤组织中发生改变。在这里，我们通过使用百分比的定性和半定量免疫光化学方法，研究了增殖性（n = 9）和分泌性（n = 9）子宫内膜以及腹膜（n = 11），卵巢（n = 20）和结直肠（it 20）子宫内膜异位症中凋亡相关蛋白p53，bc1-2，bax，p21和fas的表达。 阳性细胞和 HSCORE 分析。在子宫内膜中，p53、p21和fas表达较低，而bax和bcl-2表达升高。使用 HSCORE 分析，只有 bcl-2 表达在月经周期期间发生变化（增殖期为 48.9 +/- 34.2%，分泌期为 11.5 +/- 24.7%，p = 0.01）。使用 HSCORE 分析，卵巢子宫内膜异位症中的 p53 表达高于腹膜子宫内膜异位症 (p < 0.0001) 和结直肠子宫内膜异位症 (p = 0.03)。 P21 在卵巢子宫内膜异位症中的表达高于腹膜子宫内膜异位症 (p = 0.01) 和结直肠子宫内膜异位症 (p = 0.01)。卵巢子宫内膜异位症中的 Bcl-2 表达低于腹膜子宫内膜异位症 (p = 0.0002) 和结直肠子宫内膜异位症 (p < 0.0001)。腹膜子宫内膜异位症中的 Fas 表达高于卵巢子宫内膜异位症 (P = 0.02) 和结直肠子宫内膜异位症 (P = 0.008)。总之，这些结果证实了细胞凋亡参与子宫内膜异位症的发病机制。此外，凋亡相关蛋白的表达根据子宫内膜异位症的位置而变化，表明不同的凋亡途径参与其中。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus. Apoptosis, a physiological process by which multicellular organisms eliminate superfluous cells, is altered in tumor tissue. Here we studied the expression of the apoptosis-related proteins p53, bc1-2, bax, p21 and fas in proliferative (n =9) and secretory (n = 9) endometrium, and in peritoneal (n = 11), ovarian (n = 20) and colorectal (it 20) endometriosis, by qualitative and semi-quantitative immunolustochemical methods using the percentage of positive cells and HSCORE analysis. In endometrium, p53, p21 and fas expression was low, whereas bax and bcl-2 expression was elevated. Using HSCORE analysis, only bcl-2 expression varied during the menstrual cycle (48.9 +/- 34.2% in the proliferative phase, 11.5 +/- 24.7% in the secretory phase, p = 0.01). Using HSCORE analysis, p53 expression was higher in ovarian endometriosis than in peritoneal (p < 0.0001) and colorectal endometriosis (p = 0.03). P21 expression was higher in ovarian endometriosis than in peritoneal (p = 0.01) and colorectal endometriosis (p = 0.01). Bcl-2 expression was lower in ovarian endometriosis than in peritoneal (p = 0.0002) and colorectal endometriosis (p < 0.0001). Fas expression was higher in peritoneal endometriosis than in ovarian (p = 0.02) and colorectal endometriosis (P = 0.008). In conclusion, these results confirm the involvement of apoptosis in the pathogenesis of endometriosis. Moreover, expression of apoptosis-related proteins varies according to the location of endometriosis suggesting the involvement of different apoptotic pathways. (c) 2005 Elsevier Ireland Ltd. All rights reserved.