Measuring Lipid Packing of Model and Cellular Membranes with Environment Sensitive Probes
Measuring Lipid Packing of Model and Cellular Membranes with Environment Sensitive Probes
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DOI：
10.1021/la501226v
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发表时间：
2014-07-15
期刊：
影响因子：
3.9
通讯作者：
Simons, Kai
中科院分区：
文献类型：
--
作者：
Sezgin, Erdinc;Sadowski, Tomasz;Simons, Kai
脂质堆积的程度是生物膜的重要理化特征之一，涉及到许多膜过程。极性敏感荧光探针是测量人工膜和生物膜中膜脂堆积的常用工具。在本文中，我们系统地比较了八种不同的探针来测量膜脂有序。我们研究了这些探针在小的单层脂质体、相分离的巨大单层囊泡、细胞衍生的巨大质膜囊泡和活细胞中的表现。我们已经测试了各种可测量参数的顺序灵敏度，包括广义偏振，峰移或强度位移。我们还研究了探针的内化和光稳定性，以评估探针在延时活细胞成像中的潜力。这些结果提供了一个属性目录，以方便根据需要选择探针。
The extent of lipid packing is one of the key physicochemical features of biological membranes and is involved in many membrane processes. Polarity sensitive fluorescent probes are commonly used tools to measure membrane lipid packing in both artificial and biological membranes. In this paper, we have systematically compared eight different probes to measure membrane lipid ordering. We investigated how these probes behave in small unilamellar liposomes, phase-separated giant unilamellar vesicles, cell-derived giant plasma membrane vesicles, and live cells. We have tested the order sensitivity of a variety of measurable parameters, including generalized polarization, peak shift, or intensity shift. We also investigated internalization and photostability of the probes to assess probe potential for time-lapse live cell imaging. These results provide a catalogue of properties to facilitate the choice of probe according to need.