Polycomb repressive complex 2 attenuates ABA-induced senescence in Arabidopsis
Polycomb repressive complex 2 attenuates ABA-induced senescence in Arabidopsis
复制标题
Polycomb 抑制复合物 2 减弱 ABA 诱导的拟南芥衰老
DOI：
10.1111/tpj.14125
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发表时间：
2019-01-01
期刊：
影响因子：
7.2
通讯作者：
Zhang, Yijing
中科院分区：
文献类型：
--
作者：
Liu, Chunmei;Cheng, Jingfei;Zhang, Yijing
植物激素脱落酸（阿坝）诱导的叶片衰老促进养分再利用，并可能有助于提高植物的抗逆性。然而，过度衰老导致作物产量严重下降，衰老在不同水平上进行微调的机制仍然没有得到充分的理解。在这里，我们发现的多梳阻遏复合物2（PRC2）的核心酶的双突变体是ABA的拟南芥过敏。为了阐明阿坝和PRC2在基因组水平上的相互作用，我们广泛分析了阿坝引发的转录组和表观基因组变化。我们观察到H3K27me3优先靶向ABA诱导的衰老相关基因（SAG）。在双重，但不是单一的，突变体的PRC2酶，这些SAG被去阻遏，可以更高的诱导阿坝与野生型相比，这表明一个冗余的作用，PRC2酶在负调节ABA诱导衰老。与阿坝引发的快速转录组学变化相反，这些SAG处H3 K27 me3的减少福尔斯远远落后于其表达的诱导，表明PRC 2介导的H3 K27 me3有助于ABA诱导的衰老的长期阻尼，以防止过度敏感的反应。这项研究的结果可以作为一个范例，为全球了解之间的相互作用的快速影响的植物激素，如阿坝和长期影响的表观遗传机制在调节植物衰老过程和环境响应。
The phytohormone abscisic acid (ABA)-induced leaf senescence facilitates nutrient reuse and potentially contributes to enhancing plant stress tolerance. However, excessive senescence causes serious reductions in crop yield, and the mechanism by which senescence is finely tuned at different levels is still insufficiently understood. Here, we found that the double mutant of core enzymes of the polycomb repressive complex 2 (PRC2) is hypersensitive to ABA in Arabidopsis thaliana. To elucidate the interplay between ABA and PRC2 at the genome level, we extensively profiled the transcriptomic and epigenomic changes triggered by ABA. We observed that H3K27me3 preferentially targets ABA-induced senescence-associated genes (SAGs). In the double, but not single, mutant of PRC2 enzymes, these SAGs were derepressed and could be more highly induced by ABA compared with the wild-type, suggesting a redundant role for the PRC2 enzymes in negatively regulating ABA-induced senescence. Contrary to the rapid transcriptomic changes triggered by ABA, the reduction of H3K27me3 at these SAGs falls far behind the induction of their expression, indicating that PRC2-mediated H3K27me3 contributed to long-term damping of ABA-induced senescence to prevent an oversensitive response. The findings of this study may serve as a paradigm for a global understanding of the interplay between the rapid effects of a phytohormone such as ABA and the long-term effects of the epigenetic machinery in regulating plant senescence processes and environmental responses.