Mouse Dipeptidyl Peptidase 4 Is Not a Functional Receptor for Middle East Respiratory Syndrome Coronavirus Infection
Mouse Dipeptidyl Peptidase 4 Is Not a Functional Receptor for Middle East Respiratory Syndrome Coronavirus Infection
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DOI：
10.1128/jvi.03764-13
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发表时间：
2014-05-01
影响因子：
5.4
通讯作者：
Heise, Mark T.
中科院分区：
文献类型：
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作者：
Cockrell, Adam S.;Peck, Kayla M.;Heise, Mark T.
人类二肽基肽酶4 （hDPP4）最近被确定为中东呼吸综合征冠状病毒（MERS-CoV）感染的受体，这表明其他哺乳动物的DPP4同源物也可能支持感染。我们证明小鼠DPP4不能支持MERS-CoV感染。然而，利用小鼠DPP4作为支架，我们发现了两个调节小鼠物种特异性的关键氨基酸（A288L和T330R）。这一知识可以支持合理设计小鼠适应性MERS-CoV，以快速评估治疗方法。
Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold, we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse-adapted MERS-CoV for rapid assessment of therapeutics.