SEROLOGICAL INVESTIGATION INTO THE ASSOCIATION BETWEEN STREPTOCOCCUS-BOVIS AND COLONIC-CANCER
SEROLOGICAL INVESTIGATION INTO THE ASSOCIATION BETWEEN STREPTOCOCCUS-BOVIS AND COLONIC-CANCER
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DOI：
10.1136/jcp.46.12.1116
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发表时间：
1993-12-01
影响因子：
3.4
通讯作者：
GIBB, AP
中科院分区：
文献类型：
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作者：
DARJEE, R;GIBB, AP
目的-确定结肠癌患者中是否存在牛链球菌抗体滴度的增加，方法：用免疫印迹法和酶联免疫吸附试验（ELISA）对16例患者和16例年龄匹配的对照血清样品进行检测，以对抗两种牛链球菌和一种粪肠球菌的抗原制剂。使用免疫印迹无法区分癌症患者和对照组。ELISA确实显示牛链球菌抗体增加，但粪肠球菌抗体增加幅度更大。抗体滴度的增加是最大的抗原已被处理过的高碘酸盐，因此被认为是不是由抗体的共享D组碳水化合物antigens. Conclusion，它可能是可能的构建一个测试的基础上检测结肠癌的抗体检测牛链球菌或粪肠球菌，虽然这一概念需要相当大的进一步发展。
Aims-To determine if there was an increase in antibody titre to Streptococcus bovis in patients with colonic cancer, and if this might be a useful marker of the presence for colonic cancer.Methods-Serum samples from 16 patients and 16 age matched controls were tested by immunoblot and enzyme linked immunosorbent assay (ELISA) against antigen preparations from two strains of S bovis and one strain of Enterococcus faecalis.Results-No distinction between cancer patients and controls could be made using immunoblots. ELISA did show an increase in antibodies to S bovis, but there was a greater increase in antibodies to E faecalis. The increase in antibody titres was greatest with antigens which had been treated with periodate, and was therefore thought not to be caused by antibody to the shared group D carbohydrate antigen.Conclusion-It may be possible to construct a test for the detection of colonic cancer based on the detection of antibody to S bovis or E faecalis, though considerable further development of this concept is required.