Frequency-invariant temporal ordering of interneuronal discharges during hippocampal oscillations in awake mice
Frequency-invariant temporal ordering of interneuronal discharges during hippocampal oscillations in awake mice
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DOI：
10.1073/pnas.1210929109
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发表时间：
2012-09
期刊：
影响因子：
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通讯作者：
Csaba Varga;P. Golshani;I. Soltesz
中科院分区：
文献类型：
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作者：
Csaba Varga;P. Golshani;I. Soltesz
内源性脑节律发生在不同的频率，并与不同的行为状态提供了多尺度的时间窗口，使细胞能够以高精度的时间尖峰活动，这被认为是重要的神经元回路中的信息编码。然而，虽然GABA能输入到主细胞的特定空间域的选择性定时已知在网络振荡中起关键作用，但清醒动物中不同海马中间神经元的体内放电模式尚不清楚。在这里，我们使用了一个无麻醉，头部固定的小鼠在球形跑步机上运行或休息的录音相结合的aptacellular标记技术，以研究振荡依赖性放电的两个主要的interneuronal亚型，perisomatically投射小清蛋白阳性篮细胞（PVBCs）和远端树突投射oriens lacunosum moleculare（OLM）细胞。在θ（5-10 Hz）、γ（25- 90 Hz）、γ（“高γ”; 90-130 Hz）和涟漪（130-200 Hz）振荡期间，记录后识别的CA 1中间神经元的尖峰活动，揭示了清醒小鼠中PVBC和OLM细胞放电的细胞类型和行为状态依赖性的抑制。我们在清醒小鼠中的研究结果在几个方面与以前在麻醉动物中的神经元间放电模式的数据不同。此外，我们的研究结果表明了一种形式的频率不变，细胞类型特定的时间顺序的抑制性输入，其中PVBC衍生的体周抑制其次是OLM细胞产生的远端树突状抑制在每个网络振荡带研究，跨越超过一个数量级的频率。
Endogenous brain rhythms occurring at various frequencies and associated with distinct behavioral states provide multiscale temporal windows that enable cells to time their spiking activity with high precision, which is thought to be important for the coding of information in neuronal circuits. However, although the selective timing of GABAergic inputs to specific spatial domains of principal cells are known to play key roles in network oscillations, the in vivo firing patterns of distinct hippocampal interneurons in awake animals are not known. Here we used a combination of juxtacellular labeling techniques with recordings from anesthesia-free, head-fixed mice running or resting on a spherical treadmill to study the oscillation-dependent discharges by two major interneuronal subtypes, the perisomatically projecting parvalbumin-positive basket cells (PVBCs) and distal dendritically projecting oriens lacunosum moleculare (OLM) cells. Recordings of the spiking activity of post hoc-identified CA1 interneurons during theta (5–10 Hz), gamma (25–90Hz), epsilon (“high-gamma”; 90–130 Hz), and ripple (130–200 Hz) oscillations revealed both cell type- and behavioral state-dependent entrainments of PVBC and OLM cell discharges in awake mice. Our results in awake mice differed in several respects from previous data on interneuronal discharge patterns in anesthetized animals. In addition, our results demonstrate a form of frequency-invariant, cell type-specific temporal ordering of inhibitory inputs in which PVBC-derived perisomatic inhibition is followed by OLM cell-generated distal dendritic inhibition during each of the network oscillation bands studied, spanning more than an order of magnitude in frequencies.