Negatively Charged Carbon Nanohorn Supported Cationic Liposome Nanoparticles: A Novel Delivery Vehicle for Anti-Nicotine Vaccine.
Negatively Charged Carbon Nanohorn Supported Cationic Liposome Nanoparticles: A Novel Delivery Vehicle for Anti-Nicotine Vaccine.
复制标题
带负电荷的碳纳米角支持的阳离子脂质体纳米颗粒：一种新型的抗尼古丁疫苗递送载体。
DOI：
10.1166/jbn.2015.2156
复制
发表时间：
2015
影响因子：
2.9
通讯作者：
Zhang,Chenming
中科院分区：
文献类型：
--
作者：
Zheng,Hong;Hu,Yun;Huang,Wei;deVilliers,Sabina;Pentel,Paul;Zhang,Jianfei;Dorn,Harry;Ehrich,Marion;Zhang,Chenming
烟草成瘾是世界上第二大死亡原因。由于尼古丁（一种小分子）的性质，寻找对抗尼古丁有害影响的方法一直是社会和医学领域的一个持续挑战。本研究开发了一种基于纳米角支撑的脂质体纳米粒（NsL NPs）的新型抗尼古丁疫苗。纳米疫苗是通过使用带负电荷的碳纳米角作为用于组装阳离子脂质体的支架来构建的，阳离子脂质体允许半抗原缀合的载体蛋白缀合。组装的生物纳米颗粒是稳定的。用纳米疫苗皮下免疫小鼠，其在小鼠中诱导高滴度和高亲和力的尼古丁特异性抗体。此外，在多次给予基于NSL的抗尼古丁疫苗后，没有临床体征或全身毒性的证据。这些结果表明，基于NSL的抗尼古丁疫苗是治疗尼古丁依赖的一个有希望的候选者，并可能对戒烟有显着的贡献。
Tobacco addiction is the second-leading cause of death in the world. Due to the nature of nicotine (a small molecule), finding ways to combat nicotine's deleterious effects has been a constant challenge to the society and the medical field. In the present work, a novel anti-nicotine vaccine based on nanohorn supported liposome nanoparticles (NsL NPs) was developed. The nano-vaccine was constructed by using negatively charged carbon nanohorns as a scaffold for the assembly of cationic liposomes, which allow the conjugation of hapten conjugated carrier proteins. The assembled bio-nanoparticles are stable. Mice were immunized subcutaneously with the nano-vaccine, which induced high titer and high affinity of nicotine specific antibodies in mice. Furthermore, no evidence of clinical signs or systemic toxicity followed multiple administrations of NsL-based anti-nicotine vaccine. These results suggest that NsL-based anti-nicotine vaccine is a promising candidate in treating nicotine dependence and could have potential to significantly contribute to smoking cessation.